【作者】 曹华斌； 郭小权； 胡国良； 张彩英； 曹洪峰； 李浩棠； 黄爱民；

【Author】 CAO Hua-bin,GUO Xiao-quan,HU Guo-liang~1,ZHANG Cai-ying,CAO Hong-feng,LI Hao-tang,HUANG Ai-ming (College of Animal Science and Technology,Jiangxi Agriculture University,Nanchang 330045,China)

【机构】 江西农业大学动物科学技术学院；

【摘要】 (目的)本试验以海蓝褐蛋鸡为试验对象,研究高能低蛋白日粮中添加甜菜碱对蛋鸡肝脏中apo AI和apoB₁₀₀基因mRNA表达的影响。（方法）选300日龄海蓝褐蛋鸡135羽,随机分成3组（每组设3个重复,每个重复15羽）,分别饲喂正常日粮（对照组,代谢能11.30MJ/kg,粗蛋白17.20%）、高能低蛋白日粮（高能低蛋白组,代谢能12.81MJ/kg,粗蛋白13.04%）、添加1000 mg/kg甜菜碱的高能低蛋白日粮（甜菜碱组,代谢能12.81MJ/kg,粗蛋白13.04%）,正试期60 d,分别于试验1 d、30 d、60 d,每组选取体重相近的试验蛋鸡6羽,取肝脏,采用实时荧光定量PCR方法测定肝脏中apo A I和apo B₁₀₀基因mRNA的相对表达量。（结果）与对照组相比,饲喂高能低蛋白日粮30天可导致蛋鸡肝脏中apo A I的mRNA表达显著降低（P<0.05）,apo B₁₀₀的mRNA表达显著升高（P<0.05）,饲喂60天可导致蛋鸡肝脏中apo A I和apo B₁₀₀的mRNA表达均极显著降低（P<0.01）;与同期高能低蛋白组相比,添加1000mg/kg甜菜碱可使蛋鸡肝脏中apo A I和apo B₁₀₀ mRNA的表达水平均升高,差异显著（P<0.05）。（结论）结果提示,高能低蛋白日粮中添加甜菜碱可通过提高肝脏中apo A l和apo B₁₀₀ mRNA的表达,增强肝脏中脂类物质的转运出。更多还原

【Abstract】 (Objective) The experiment was conducted to investigate the influence of high-energy low-protein diet supplemented with betaine on apo AⅠand apo B100 mRN A expression in liver of laying hens.（Method） One hundred and thirty five birds（Hy-line Variety Brown laying hens,Gallus domesticus） aged 300 days were randomly allotted into 3 dietary treatments of 45 broilers each.The groups were:1) Control group（CG,semisynthetic commercial layer standard diet in accordance with the nutrient requirements of poultry（National Research Councile,1998）,ME 11.30MJ/kg and CP 17.20%）; 2) high energy-low protein group（HELPG,high energy-low protein diet which were partially formulated in accordance with Controls,ME 12.81 MJ/kg and CP 13.04%）;3) betaine group（BG,the high energy-low protein diet supplemented with 1000 mg betaine/kg DM,ME 12.81 MJ/kg and CP 13.04%）.All birds were reared for 60 days under normal condition in the Jiangxi Agriculture University Poultry form and were fasted overnight with a free access to water before sacrificed.Apo A I and apo B₁₀₀ mRNA expression in liver of six layers（stratified by weight,1.6-1.7 kg ） from each group were recorded by real-time fluorescent quantitative PCR on day of 1st,30^th and 60^th.（Result） The results showed that compared with the CG, the level of apo A I mRNA expression in liver of layers fed with high energy-low protein diet decreased significantly on the day of 30（P<0.05）,while the level of apo B₁₀₀ mRNA expression increased significantly（P<0.05）.After 60 days fed with the high energy-low protein diet,the level of apo A I and apo B₁₀₀ mRNA expression were decreased very significantly （P<0.01）.Compared with the same period of HELPG,the high energy-low protein diet supplemented with 1000 mg betaine/kg DM could result in the increasing significantly of the levels of apo A I and apo B₁₀₀ mRNA expression in liver of layers（P<0.05）.（Conclusion） These results indicated that betaine could protect the laying hens from fatty liver induced by high energy-low protein diet through increasing the expression levels of liver apo A I and apo B₁₀₀ mRNA,and thus enhancing the transportation of lipids from the liver.更多还原