【作者】 顾建红； 王世涛； 刘学忠； 卞建春； 袁燕； 熊桂林； 刘宗平；

【Author】 Gu Jian-hong,Wang Shi-tao,Liu Xue-zhong,Bian Jian-chun,Yuan Yan,Xiong Gui-lin,Liu Zong-ping~* (College of Veterinary Medicine,Yangzhou University,Yangzhou 225009)

【机构】 扬州大学兽医学院；

【摘要】 【目的】探讨不同浓度1α,25-（OH）₂D₃(0、10^-9、10^-8、10^-7mol/L)对体外培养SD大鼠成骨细胞（osteoblasts,OB）内巨噬细胞集落刺激因子（M-CSF）mRNA表达以及M-CSF对破骨细胞（osteoclasts,OC）增殖与分化的影响。【方法】lα,25-（OH）₂D₃与OB共培养24、48、72 h,采用FQ-PCR法测定M-CSF mRNA表达量。M-CSF与骨髓单核细胞共培养9 d,抗酒石酸酸性磷酸酶（TRAP）染色观察并计数OC量,扫描电镜观察象牙片吸收陷窝。【结果】与对照组比较,不周浓度1α,25-（OH）₂D₃作用48、72 h均显著（P<0.05）或极显著（p<0.01）促进OB内M-CSF mRNA的表达;同时,M-CSF较对照组促进骨髓单核细胞贴壁与增殖,并能与RANKL联合诱导OC的形成与活化。【结论】1α,25-（OH）₂D₃能上调OB内M-CSFmRNA的表达,间接促进OC的生成和活化,增强骨更新。更多还原

【Abstract】 [Objective]To investigate the expression of M-CSF mRNA,osteobalsts obtained from Sprague Dawley rat were treated with different concentrations of 1α.25-（OH）₂D₃(0,10^-9,10^-8,10^-7 mol/L).Meanwhile,to investigate the effects of M-CSF on osteoclasts’ proliferation and differentiation,bone marrow mononuclear cells（BMM） were cultured with or without M-CSF. [Method]The expression of M-CSF mRNA were determined through FQ-PCR.The formation of OC were detected by histochemical staining for tartrate-resistant acid phosphatase（TRAP）,and the detection of lacunar resorption via scanning electron microscopy at the 9^th day.[Result]Compared with the control group,different conentrations of 1α,25-（OH）₂D₃ can significantly or very significantly induce the expression of M-CSF mRNA（P< 0.05 or P< 0.01）.M-CSF can promote BMM adherence and proliferation,and it can induce formation and activation of osteoclasts combined with RANKL.[Conclusion]1α,25-（OH）₂D₃ can enhance bone turnover through facilitating the generation and activity of osteoclasts via up-regulation of M-CSF mRNA.更多还原