【作者】 温立斌； 何孔旺； 杨汉春；

【Author】 WEN Li-bin~1,HE Kong-wang~1,YANG Han-chun~2 (1.Jiangsu Academy of Agricultural Science,Nanjing 210014;2.China Agricultural University,Beijing 100094)

【机构】 江苏省农业科学院； 中国农业大学；

【摘要】 研究目的:确定猪体内存在与猪圆环病毒2型核苷酸序列高度同源的因子;方法:首先利用PCR方法对来自临床表现为猪断奶后多系统衰竭综合征的猪血清所抽提的DNA进行扩增,根据获得的序列再设计引物进行反向PCR,拼接得到类猪圆环病毒2型因子P1的全基因组序列,最后根据P1全基因组序列设计引物扩增其全基因组加以验证;结果:首次完成了类猪圆环病毒2型因子P1的全基因组序列测定。测序结果表明P1因子为环状DNA基因组,全长648个核苷酸,包括3个开放阅读框。除5’端16个核苷酸外,P1因子与国内猪圆环病毒2型BF毒株的核苷酸同源率为98.42%。系统进化分析表明P1与猪圆环病毒有很近的亲缘关系;结论:猪体内存在类猪圆环病毒2型因子P1。更多还原

【Abstract】 Objective The aim is to discover a agent containing high homologous sequences of porcine circovirus type 2 in swine;Method PCR was developed for detection of DNA extracted from porcine sera collected from pigs with clinical signs of postweaning multisystemic wasting syndrome,then a reverse PCR was carried out based on the acquired sequences in this study,the sequences of PCR fragments were assembled into consecutive sequence and then finally verified by amplifing the agent complete genome;Results Complete nucleotide sequence of genome of porcine circovirus type 2-like agent P1 was first reported in this paper,P1 contained circular genomic DNAs of 648 nucleotides and three open reading frames.Except 16 nucleotides in the 5’ end of the genome,the genome of P1 showed 98.42%of nucleotide homology compared with porcine circovirus type 2 BF isolate.Phylogenetic analysis revealed P1 was closely related to the porcine circovirus;Conclusion Results suggested that there existed porcine circovirus type 2-like anget P1 in swine.更多还原