【作者】 田长永； 杨景晁； 马泽芳； 宋连喜； 范强； 俞美子；

【Author】 Tian Changyong~1,Yang Jingchao~2,Ma Zefang~2,Song Lianxi~1,Yu Mmeizi~1,Fan Qiang~1 1 Liaoning Agricultural Vocational-Technical College,Yingkou 115009;2 College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109

【机构】 辽宁农业职业技术学院； 青岛农业大学动物科技学院；

【摘要】 为了进一步探索辽宁绒山羊卵母细胞体外成熟的方法,本试验以屠宰场绒山羊卵巢为材料,采用抽吸法收集直径大于2mm卵泡的卵母细胞,研究卵丘细胞对卵母细胞体外成熟和孤雌发育的影响。试验1,将一部分COCs经机械吹打脱除卵丘细胞成为机械裸卵（DOs）,然后以4种方式培养,即COCs单独培养、DOs与COCs共培养DOs（COCs）、DOs与卵丘颗粒细胞共培养DOs（CCs）,以及DOs单独培养。试验2,根据包裹卵母细胞的卵丘细胞的完整性分为三组,一组有3层卵丘细胞紧密包围的卵丘细胞复合体COCs₃,有1-3层卵丘细胞包围的卵母细胞记为COCs_1-3,无卵丘细胞包围的裸露卵母细胞NOs,3组各自单独培养。结果表明:COCs组的成熟率、孤雌卵裂率和囊胚率最高,分别为83.25%、41.75%和29.25%,卵丘细胞的存在有利于卵母细胞体外成熟和随后的发育,COCs₃组成熟率、孤雌卵裂率和囊胚率分别为83.25%、42.50%和29.75%,显著高于COCs_1-3组和NOs组,卵丘细胞的完整性也影响着卵母细胞的体外发育,自然裸卵已失去体外发育能力。更多还原

【Abstract】 For explore the method of of in vitro matutation of liaoning cashmere goats oocytes,this experiment was carried out to study effects of of cumulus cells on maturation in vitro and pathenogenetic development of oocytes.The oocytes were recovered directly from the oocytes of follicles,>2 mm in diameter.Experiment 1, DOs（denuded oocytes） were obtained from COCs freed of whole CCs,and then the oocytes were cultured in the following four ways.（1）COCs were cultured alone;（2）DOs were co-cultured with COCs（DOs（COCs））;（3）DOs were co-cultured with CCs（DOs（CCs））;（4）DOs were cultured alone.Experiment 2,we evaluated effect of different layers of cumulus cells on the maturative ability and parthenogenetic development of oocytes.These oocytes were divided into COCs₃,COCs_1-3 and NOs according to the layers of cumulus cells.We found that there was significate difference in maturation and cleavage rates between ooctyes invested by different layers of cumulus cells,and the oocytes enclosed with more layers of cumulus cells,both maturation and cleavage rates were higher than oocytes enclosed with fewer layers of cumulus cells.The DOs（COCs） was better than DOs（CCs）,and the NOs was a kind of degenerate ooctyes.Our findings would be helpful to the choice of recipient oocytes for embryonic technologies which will need high quality oocytes.更多还原