【作者】 凌泽继； 黎金秀； 秦津； 潘堂峰； 宋小白； 杨柄壮； 许典新； 韩涛； 潘琼； 李瑞明； 谢莹雪； 周辰瑜；

【Author】 LIN Ze-ji,LI Jin-xiu,QIN Jin,PAN Tang-feng,SONG Xiao-bai,YANG Bin-zhuang,XU Dian-xin,HAN Tao,PAN Qiong,LI Rui-ming,XIE Yin-xue,ZHOU Zhen-yu (College of Animal Science & Technology,Guangxi University,Nanning,Guangxi 530004)

【机构】 广西大学动物科技学院； 广西隆安县水产畜牧兽医局； 中国农业科学院(广西)水牛研究所； 广西壮族自治区畜禽品种改良站；

【摘要】 本研究采用免疫组织化学SuperPicTureTM二步法检测5头青年摩杂一代水牛下丘脑、垂体和卵巢中促性腺激素释放激素(GnRH)的分布情况,旨在研究GnRH在下丘脑、垂体及卵巢的分布上的联系,进而了解GnRH在下丘脑、垂体及卵巢三者之间分泌释放途径,为进一步研究GnRH在生殖中的生理和作用机制提供形态学依据,并对青年摩杂一代水牛的繁殖育种起到一定的参考指导作用。试验结果表明:1、GnRH存在于青年摩杂一代水牛下丘脑-垂体-卵巢轴的各个环节。2、青年摩杂一代水牛下丘脑中各主要核团都有GnRH免疫阳性神经元的分布,尤其以视上核、视前核、室旁核分布较多。3、GnRH免疫阳性产物只存在青年摩杂一代水牛的腺垂体中。4、青年摩杂一代水牛卵巢中生殖上皮细胞、颗粒细胞和黄体细胞发现有GnRH免疫阳性产物。更多还原

【Abstract】 To study the association of gonadotropin releasing hormone(GnRH)’s distribution in hypothalamatic、pituitary and ovary,and understand how the GnRH secrete releasing in hypothalamus、hypophysis and ovaries,the paraffin section、Nissl staining、H-E staining and immunohistochemistry SuperPicTureTM two step method were used to detect the distribution of GnRH in hypothalamatic-pituitary-ovary axis of five femal murrah hybrid water bufflos.The test results could provide morphology according to study the GnRH’s synthesis and mechanism of action,and could play reference and directions part in breeding murrah hybrid water bufflo.The test results show that:l、The GnRH was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of murrah hybrid water bufflo.2、The GnRH immunoactive neurons were first discovered in every main nucleus of murrah hybrid water bufflo hypothalamus,especially distributed in supraoptic nucleus、preoptic nucleus、paraventricular nucleus most.3、The GnRH immunoactiveproductions were first discovered only in murrah hybrid water bufflo adenohypophysis.4、The GnRH immunoactive productions were first discovered in the germinal epithelium cells、granulosa cells and lutein cells of murrah hybrid water bufflo.更多还原