【作者】 文明； 毛君婷； 史开志； 周碧君； 王开功；

【Author】 WEN-ming~2,MAO Jun-ting~1,SHI Kai-zhi~1,ZHOU Bi-jun~(2*),WANG Kai-gong~2 (1.College of Animal Science,Guizhou University,Guizhou Guiyang 550025 China; 2.Institute of animal disease,Guizhou University,Guizhou Guiyang 550025,China)

【机构】 贵州大学动物科学学院； 贵州大学动物疫病研究所；

【摘要】 为了解2007年4月至2008年7月贵州省猪繁殖与呼吸综合征(PRRS)分子流行病学特征,在此期间基于Nsp2基因在全省33个发病猪场展开PRRSV调查,结果表明有3种不同Nsp2基因缺失类型毒株在贵州省流行.参考PRRSV经典毒株相应序列,根据Nsp2基因缺失的不同情况将PRRSV贵州省流行毒株分为3种类型,类型Ⅰ(3/33)缺失177个碱基,类型Ⅱ(29/33)缺失90个碱基,类型Ⅲ(1/33)缺失93个碱基.类型Ⅱ毒株与近年国内广泛流行的高致病性PRRSV缺失情况一致,类型Ⅰ和Ⅲ是2种新发现的缺失型毒株,但其系统发生地位与高致病性PRRSV接近.更多还原

【Abstract】 To recognize the molecular epidemiology feature of PRRS in Gguizhou province during April 2007 to July 2008, analisysed the Nsp2 gene sequences of 33 strains of PRRSV from 33 infected pig farms in Gguizhou province this period.The resultes indicate 3 types of PRRSV prevaienced in Guizhou province.Compared with the classical stains and according to the Nsp2 gene delection difference,divided the 33 strains as 3 types,typeⅠ(3/33)has 177bp deletion,typeⅡ(29/33)has 90bp,typeⅢhas 93bp.The typeⅡstrains have the same deletion form with the high pathogenic PRRSV in China,both typeⅠand typeⅢare 2 new deletion types of PRRSV,but the phylogenetic relationship between the 2 new types and high pathogenic PRRSV is near.更多还原