【作者】 崔恒敏； 崔伟； 李敏； 彭西； 邓俊良；

【Author】 CUI Heng-min CUI Wei LI Min PENG Xi DENG Jun-liang (Key Laboratory of Environmental Hazard and Animal Diseases of Sichuan Province, College of Veterinary Medicine,Sichuan Agricultural University,Ya’an,Sichuan,625014,China)

【机构】 环境公害与动物疾病四川高校重点实验室 四川农业大学动物医学院；

【摘要】 本试验旨在研究高铜对雏鸡肾脏组织结构及生化指标的影响.360只1日龄艾维茵肉鸡随机分为6组,分别喂以对照日粮(Cu 10.89mg/kg)和高铜日粮(Cu 100mg/kg,高铜Ⅰ组;Cu 200mg/kg,高铜Ⅱ组;Cu 400mg/kg,高铜Ⅲ组;Cu 600mg/kg,高铜Ⅳ组;Cu 800mg/kg,高铜Ⅴ组)6周.与对照组比较,高铜Ⅲ组、高铜Ⅳ组、高铜Ⅴ组雏鸡肾脏出现不同程度的病理损害,肾小管上皮细胞颗粒变性、空泡变性;超微结构观察,肾小管上皮细胞线粒体肿胀,嵴断裂甚至溶解消失呈空泡状.同时,高铜Ⅲ组、高铜Ⅳ组、高铜Ⅴ组雏鸡肾脏及血清铜含量显著升高(P<0.01),肾脏铜锌超氧化物歧化酶(CuZn-SOD)活性显著降低(P<0.01),丙二醛(MDA)含量和羟自由基(-OH)含量显著升高(P<0.01).结果表明,日粮铜含量400mg/kg及其以上可引起雏鸡肾脏组织的病理损伤以及肾脏抗氧化功能的降低,导致肾脏功能降低.更多还原

【Abstract】 The experiment was conducted with the objective of examining the effect of dietary high copper on the renal tissue structure and biochemical parameters in chickens.360 one-day-old Avian chickens were randomly divided into six groups and feed on diets as follows:controls(Cu 10.89 mg/kg)and five high copper groups for six weeks.Injuries were appeared in high copper groupsⅢ,ⅣandⅤwhen compared with the control group,there were granular degeneration and vacuolar degeneration in the renal tubule epithelial cells.And,the renal copper contents and serum copper contens were much higher in high copper groupsⅢ,ⅣandⅤthan in control group(P<0.01).Also,in high copper groupsⅢ,ⅣandⅤ,the renal malondialdehyde and hydroxy radical contents were increased(P<0.01),and the renal cuprozinc-superoxide dismutase(CuZn-SOD)activities were decreased(P<0.01)compared with those of control group.The result showed that dietary copper in excess of 400mg/kg caused pathological injures and the antioxydic function impaired in kidney.The renal function was finally depressed.更多还原