【作者】 崔恒敏； 李敏； 彭西； 崔伟； 柏才敏； 邓俊良；

【Author】 CUI Heng-min LI Min PENG Xi CUI Wei BAI Cai-min DENG Jun-liang (Key Laboratory of Environmental Hazard and Animal Diseases of Sichuan Province, College of Veterinary Medicine,Sichuan Agricultural University,Ya’an,Sichuan,625014,China)

【机构】 环境公害与动物疾病四川高校重点实验室 四川农业大学动物医学院；

【摘要】 1日龄艾维茵肉鸡健雏360羽,随机分为6组,分别喂以对照组日粮(10.89mg/kg)和高铜日粮(Cu 100 mg/kg,高铜Ⅰ组;Cu 200 mg/kg,高铜Ⅱ组;Cu 400 mg/kg,高铜Ⅲ组;Cu 600 mg/kg,高铜Ⅳ组;Cu 800 mg/kg,高铜Ⅴ组)6w,观察高铜对雏鸡脑组织和血清一氧化氮(N0)含量及一氧化氮合酶(NOS)活性变化的影响,探讨高铜对雏鸡脑组织的损伤机理.高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组脑组织和血清NO的含量较对照组显著或极显著升高(p<0.05或p<0.01).NOS的活性随日粮铜含量的升高而升高,各高铜组脑组织NOS的活性较对照组极显著升高(p<0.01);高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组血清NOS的活性较对照组显著或极显著升高(p<0.05或p<0.01).日粮铜水平在200mg/kg及以上时,脑组织和血清NO的含量和NOS的活性随日粮铜的含量升高而升高,脑组织因氧化而受损.更多还原

【Abstract】 The experiment was conducted with the objective of approach the effect of dietary high copper on the nitrogen monoxide(NO)content and nitric oxide synthase(NOS)activity in brain tissue and serum in chickens by the biochemical methods.360 one-day-old Avian chickens were randomly divided into six groups,and fed on diets as follows:controls(Cu 10.89 mg/kg)and high copper(Cu 100 mg/kg,high copper groupⅠ;Cu 200 mg/kg,high copper groupⅡ;Cu 400 mg/kg,high copper groupⅢ;Cu 600 mg/kg,high copper groupⅣ;Cu 800 mg/kg,high copper groupⅤ)for six weeks.The NO content and NOS activity in brain tissue were much higher in high copper groupsⅡ,Ⅲ,ⅣandⅤthan in control group(p<0.05 or p<0.01).At the same time,changes of the serum NO content and NOS activity were consistent with those of the brain tissue.These results showed that the NO content and NOS activity were greatly increased under the dietary copper excess of 200mg/kg.更多还原