【作者】 王彦红； 陈素娟； 刘武杰； 仇旭升； 董丽； 彭大新； 刘秀梵；

【Author】 WANG Yan-hong,CHENG Su-juan,LIU Wu-jie,QIU Xu-shen,DONG Li,PENG Da-xin,LIU Xiu-fan~* (Animal Infectious Disease Laboratory,College of Veterinary Medicine, Yangzhou University,225009 Yangzhou,China)

【机构】 扬州大学畜禽传染病学农业部重点开放实验室；

【摘要】 作者旨在探讨鸡痘病毒ORF073或ORF214基因缺失后,在母源抗体存在情况下对重组病毒免疫效力的影响.将构建好的在ORF073或ORF214基因插入H5亚型AIV HA基因的重组鸡痘病毒(rFPVLP-Δ73LRH5A、rFPVLP-Δ214LRH5A)及单表达H5亚型AIV HA基因的重组鸡痘病毒(rFPVLP-12LSH5A)分别免疫SPF鸡和商品鸡,检测重组疫苗诱导的免疫效力.结果:3种重组鸡痘病毒在SPF鸡产生较高的HI抗体效价及100%的免疫保护;在HI母源抗体效价为2.45的商品鸡体内基因缺失株重组病毒比rFPVLP-12LSH5A易于清除,抗体上升缓慢而且免疫28 d后HI抗体效价较低,免疫保护率低,分别为16.7%和23.3%;在母源HI抗体效价为0的商品鸡体内基因缺失株重组病毒免疫后产生的抗体效价高,免疫28 d后分别达到3.50和3.17.结果表明在商品鸡体内母源抗体影响下,缺失ORF073或ORF214基因的重组鸡痘病毒产生的免疫效力降低.更多还原

【Abstract】 To assess the role that ORF73 or ORF214 gene of fowlpox virus（FPV）played in the regulation of immune response against FPV in commercial chickens with maternal antibodies,two recombinant fowlpox viruses（rFPV）that FPV073 or FPV214 genes inserted and coexpressed the influenza haemagglutinin(rFPV_LP-Δ73LRH5A,rFPV_LP-/Δ214LRH5A)and the parental recombinant virus expressing the influenza haemagglutinin(rFPV_LP-12LSH5A)were constructed.These viruses were assessed for their immunological efficacy on SPF chickens and commercial chickens.The result showed the high titer of HI antibodies was induced by three rFPV,and the protective efficacies were universal 100%in SPF chickens.However,the low HI titer was induced by rFPV_LP-/Δ73LRH5A or rFPV_LP-Δ214LRH5A in commercial chickens with HI maternal antibodies up to 2.45,the protective efficacies were 16.7%and 23.3%,respectively.At the same time,rFPV with deleted ORF073 or ORF214 genes in vivo were easier to eliminate than rFPV_LP-12LSH5A.On the other hand,in the inoculated commercial chickens without HI maternal antibodies with rFPV_LP-Δ73LRH5A or rFPV_LP-Δ214LRH5A,the HI antibody titers increased and approached to 3.50 and 3.17, respectively after 28 days.Consequently,maternal antibodies depressed immune efficacy by rFPV with deleted ORF073 or ORF214 genes(rFPV_LP-Δ73LRH5A,rFPV_LP-Δ214LRH5A)induced.更多还原