【作者】 李勇； 夏志平； 刘雯； 张宁； 屈雪琪； 白雪； 梅林； 高英杰；

【Author】 Li Yong~(1,2),Xia Zhi ping~2,Liu Wen~(1,2),Zhang Ning~(1,2),Qu Xue qi~1,Bai xue~1,Mei lin~1,Gao Ying jie~1 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China; 2.Military Veterinary Institute,Academy of Military Sciences or PLA,Changchun,Jilin,130062,China)

【机构】 解放军军事医学科学院军事兽医研究所； 吉林大学畜牧兽医学院；

【摘要】 将布氏杆菌分不同剂量皮下注射接种于健康的牛,于接种后45d剖杀,采集相关的器官组织制备组织切片,应用免疫组化方法检查细菌在牛体内的分布.结果显示:肝脏、脾脏、肾脏、淋巴结出现强阳性信号;在高剂量注射的牛体内,肺脏,颌下淋巴结出现阳性细胞;心和健康牛组织呈阴性反应.阳性信号主要位于感染细胞的胞质中,偶尔出现在细胞核内.更多还原

【Abstract】 The healthy conventional cattles were inoculated subcutaneouly with different CFU of Brucella abortus strain,the cattles were necropsy on day post inoculation.Tissues were collected for preparing tissue sections and immunohistochemistry protocol were developed to detect distribution of B.abortus bacterium in tissue samples.Results showed that in Liver,Spleen,Kidney and lymphoid tissues were positive for B.abortus antigen.Positive signals also presented in lung,Mandibular lymph node.Other tissues such as heart and control were negative for antigen.B.abortus antigens were localized predominately in the cytoplasm of the infected cells and rarely observed in the nucleus of the cells.更多还原