【作者】 杨宏军； 李旭妮； 何洪彬； 高运东； 毛开荣； 仲跻峰； 丁家波；

【Author】 Yang hongjun~1 Li xuni~2 He hongbin~1 Gao yundong~1 Mao kairong~2 Zhong jifeng~(1*) Ding jiabo~(2*) (1:Milch Cow Research Center of Agricultural Science Academy of Shandong Province Jinan,Shandong 250100,China; 2 China Institute of Veterinary Control,Beijing 100081,China)

【机构】 山东省农业科学院奶牛研究中心； 中国兽医药品监察所；

【摘要】 本实验目的是观察奶牛在布鲁氏菌S19疫苗免疫状态下血清凝集抗体的动态规律和免疫后奶牛对布鲁氏菌水解素的皮肤迟发性变态反应差异,并探索两者的相关性。实验选择布病阴性的性成熟青年黑白花奶牛50头,注射S19疫苗1×109CFU/头,定期采集血液测抗体水平,第60天用布鲁氏菌水解素皮内注射,检测变态反应。结果显示所有免疫牛均能在15d时检测到凝集抗体,30d达到峰值。迟发性变态反应表明免疫牛对布鲁氏菌水解素具有良好的敏感性,变态反应强度与抗体峰值水平存在相关性,但差异不显著,相关系数为0.235。更多还原

【Abstract】 The object of this experiment was to observe the agglutinating antibody variation in serum of dairy cow vaccined with brucella S19,and get the correlation between the agglutinating antibody variation and the brucellin skin allergy.Fifty sexual maturity youth cattle without brucelsis were chosen to be vaccined with the S19 in dose of 109CFU.Blood was collected regularitily to check the agglutinating antibody level of the serum.To analysis the skin allergy of the dairy cattle, brucellin was injected intradermally to all the vaccined cattle.Result of the experiment displayed that the agglutinating antibody were inspected in serum of all the vaccined cattle on the 15d,and moderately breakdowned after a peak on 30d.The tardive allergy of the catle on brucellin showed a favourable sensitivity.The correlation of the agglutinating antibody variation in Serum and the brucellin skin allergy was not notable,with a correlation coefficient of 0.235.更多还原