【作者】 王旋； 黄兵； 张训海； 于可响； 钟登科； 宋敏训； 龚争； 赵磊；

【Author】 Wang xuan~1 Huang bing~2 Zhang xunhai~(1*) Yu kexiang~2 Zheng dengke~1 Song minxun~2 Gong Zheng~1 Zhao Lei~1 (1.Anhui Key Laboratory of Poultry Infectious Disease Prevention And Control,Fengyang 233100,China 2.Shandong Institute of Poultry Science,Jinan 250100,China)

【机构】 家禽疫病防控监测安徽省重点实验室； 山东省农业科学院家禽研究所；

【摘要】 分别对源于安徽某地同期分离鉴定的鸡源、鸭源、鹅源NDV致病株（WF00C、WF00D、WF00G）的HN基因进行RT-PCR扩增、克隆和序列测定,并对其核苷酸和推导氨基酸序列进行分析。结果显示,各分离株HN基因均含1个完整的开放阅读框（ORF）,全长为1716bp,编码571个氨基酸,有5个潜在的糖基化位点,13个半胱氨酸（Cys）残基。WF00D株与WF00C株、WF00G株以及Genebank下载的16株各基因型代表株的HN基因编码区的同源性进行比较,其核苷酸序列同源率为82.1～99.6%,推导的氨基酸序列同源率为88.1～99.3%;三个不同禽源分离株间的同源性较高,其中鸭源、鹅源同源性最高,并与ZJ1、SF02和NA₁等鹅源毒株和鸭源毒株FP1/02之间差异较小。HN基因系统发育进化树分析显示,该三个不同禽源NDV致病性分离株均归为基因Ⅶ型。更多还原

【Abstract】 The whole sequence of HN gene of avian paramyxovirus isolates WF00C,WF00D,WF00G from Anhui was amplified by reverse transcription-polymerase chain reaction（RT-PCR）,and further cloned into pMD18-T vector,and positive recombinant plasmids were obtained by polymerase chain reaction（PCR）.The sequence analysis showed they had a single opening read frame（ORF）,the nucleotide sequence of the HN gene were 1716bp in length and which encoded polypeptide of 571 animo acid,contained 5 potential asparagine_linked glycosylation sites and 13 cysteine（Cys） residues.Comparison of HN gene nucleotide sequences of WF00D strain with 16 other published sequences from Genebank showed that the homology of the nucleotide was 84.7%～99.6%,the homology of the deduced amino acid sequences was 88.1%～99.3%.Three isolates from Anhui appeared higher homology each other,the highest homology between WF00D and WF00G,there is only a lilltle diffrence that compared with ZJ1、SF02、NA₁ from goose and FP1/02 from duck too.The results of phylogenetic tree showed that three different pathogenic avian paramyxovirus isolates were classified as genotypeⅦ.更多还原