【作者】 成子强； 张利； 刘思当； 张玲娟； 崔治中；

【Author】 CHENG Zi-qiang~*,ZHANG Li,LIUSi -dang,ZHANG Ling-juan,CUI Zhi -zhong (Animal Science andTechnology College ofShandongAgricultural University,Taian271018,China)

【机构】 山东农业大学动物科技学院；

【摘要】 首次报道了中国特有鸡种--麻鸡J亚群白血病的发病情况。山东某种鸡场饲养的中国麻鸡,于开产前出现消瘦、贫血、瘫痪等症状,死亡率达10%。经大体剖检发现,病鸡的内脏器官均弥漫性肿大,色彩斑驳,质度较硬;在胸骨内侧、小肠浆膜面和气管粘膜面出现大小不等的肿瘤结节,呈灰白色。组织学检查发现,增生的肿瘤细胞为均一的髓细胞。用禽白血病病毒J亚群(ALV-J)的特异性引物进行PCR检测,阳性率为89%(15/17);PCR产物测序,其基因序列、预期氨基酸序列与ALV-J原型株HPRS-103的同源性分别为98.05%和97.4%。用ALV-J单克隆抗体,经免疫组织化学检测发现,在肿瘤组织、肝、脾、肾、骨髓、腺胃中呈现强特异性染色。上述检测表明此髓细胞肿瘤是由ALV-J感染引起的。ALV-J麻鸡病例的发现警示:应注意中国地方种鸡的白血病净化工作。更多还原

【Abstract】 Myeloid leukosis(ML) cases were first diagnosed in a chicken flock of Chinese local breed in Shan dong province. The main symptom includedwasting,weight loss,anemia.It caused about10%mortalityof about15000 birds at the age of 120 - day.In the necropsy,gray - white nodules and protrusions in various sizes were commonly observed on the surface of the sternum,intestine and trachea.Almost all viscera tissues showed moderate to severe enlargementwith diffuse gray - white nodules.Histological examination indicated that the tumor cells proliferated in tissueswere myelocytes with eosinophilic granules in cytoplasm.In PCR with a pair of ALV -J -specific primers,15 of 17 liver samples were positive.PCR product of one positive sample was sequenced and demonstrated 98.05%and 97.4%identitywith ALV -J HPRS - 103 strain at nuclei acid and amino acids level,respectively.By immunohistochemistry (IHC) technique with ALV -J monoclonal antibody,the most intense stainingwas in the tumortissue,liver,spleen,kidney,bone marrow,and proventriculus.The results indicate that ALV -J already caused chickens infection and dead in Chinese local breed.更多还原