节点文献

外源性激素模拟犬发情周期的研究

Artificial simulation of oestrus cycle with exogenous hormone in the bitch

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 秦清明程浩周劲松李德如刘宇黄群山

【机构】 华南农业大学兽医学院

【摘要】 本实验旨在通过外源性激素模拟犬发情周期,探讨阴道上皮细胞检查确定发情周期阶段的可行性。实验犬卵巢子宫切除45天后,依据发情周期中雌激素和孕酮的变化规律,肌肉注射苯甲酸雌二醇和黄体酮,人工模拟犬的发情周期,观察实验犬的临床表现和阴道上皮细胞的变化。结果发现,人工模拟发情周期各期实验犬的临床表现与犬在自然发情周期各期的表现相似;表层细胞所占比例在乏情期最低,为14.33±3.65%;在发情前期表层细胞所占比例随着雌激素的出现和水平升高而增加,在发情期达到最高值92.28±4.27%,在发情后期又随着雌激素水平降低和消失而降低。这些结果表明,阴道上皮细胞变化能反映体内雌激素的变化,阴道上皮细胞的变化可以用来区分和鉴定犬的乏情期、发情前期、发情期和发情间期。

【Abstract】 To study on the validity of vaginal cytology to determine oestrus cycle,seven bitches ovariohysterectomized 45 days before were treated with intermuscular injection of estradiol benzoate and progesterone to simulate the patterns of estrogen and progesterone in bitches’ oestrus cycle.Clinical signs were recorded and vaginal epithelial cell smears were evaluated.The clinical signs were were similar in the artificial oestrus cycle to those in natural oestrus cycle.The vaginal cytology during the artificial oestrus cycle revealed that superficial cell percent was lowest 14.33±3.65%in anestrus, superficial cell percent evidently increased following the increase of estradiol level in prooestrus and reached highest 92.28±4.27%in estrous,then superficial cell percent obviously decreased following the decrease of estradiol level in diestrus. These results showed that vaginal cytology was an effective index of estradiol level and could be adopted to distinguish anestrus,proestrus,estrus and diestrus in bitches.

【关键词】 发情周期阴道上皮细胞
【Key words】 oestrus cyclevaginal epithelial cellcanine
  • 【会议录名称】 中国畜牧兽医学会兽医产科学分会第五届全体会议第十次学术研讨会论文集
  • 【会议名称】中国畜牧兽医学会兽医产科学分会第五届全体会议第十次学术研讨会
  • 【会议时间】2009-10-01
  • 【会议地点】中国河南郑州
  • 【分类号】S829.2
  • 【主办单位】中国畜牧兽医学会兽医产科学分会
节点文献中: 

本文链接的文献网络图示:

本文的引文网络