【作者】 齐桂平； 胡月婷； 杨玉杉； 陈杰； 曹炜； 郑建斌；

【Author】 Qi-guiping~1,Hu-yueting~2,Yang-yushan~2,Chen-jie~2,Cao-wei~(1,2),Zheng-jianbin~1 (1.Institute of Analytical Science/Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University,Xi’an 710069,China;2.Department of Food Science and Engineering, Northwest University,Xi’an 710069,China)

【机构】 西北大学分析科学研究所/陕西省电化学重点实验室； 西北大学食品科学与工程系；

【摘要】 本文以油菜蜜为研究对象,建立了一种同时测定油菜蜜中五种酚类化合物的HPLC-ECD分析方法。采用高效液相色谱电化学检测法,以甲醇（A）和2%醋酸（B）)水溶液为流动相,梯度洗脱,其梯度洗脱程序为0min时A-B（2:98）,6min时A-B（8:92）,10min时A-B（15:85）,15min时A-B（35:65）,20min时A-B（55:45）,25min时A-B（70:30）,30min时A-B（70:30）;流速为1.0mL/min;电化学检测器的工作电位为0.8V;柱温为30℃。五种酚类化合物的浓度分别在6.83×10^-2～5.46μg/mL,9.6×10^-2～8.67μg/mL,1.05×10^-1～12μg/mL,9.1×10^-2～7.3μg/mL,1.63×10^-1～13μg/mL范围内与各自的峰面积呈良好的线性关系（r>0.9997）。样品的加样回收率分别为97.4%,98.6%,99.1%,101.6%,99.3%。该方法是一种简单快速、灵敏、准确的分析方法,可用于油菜蜜的质量评价。更多还原

【Abstract】 To establish a high performance liquid chromatography-electrochemical detection（HPLC-ECD） method for determination of five phenolic components in rape honey.HPLC-ECD was used.The separation was performed by gradient elution with methanol and 2%acetic acid（2%A-98%B at 0 min,8%A-92%B at 6 min,15%A-85%B at 10 min, 35%A-65%B at 15 min,55%A-45%B at 20 min,70%A-30%B at 25min,70%A-30%B at 30 min） as mobile phase at a flow rate of 1.0mL/min.The detection was done at 0.8 V,and column temperature is 30℃.All calibration curves of the five phenolic compounds showed good linearity（r>0.9997） within the test ranges,6.83×10^-2～5.46μg/mL,9.6×10^-2～8.67μg/mL,1.05×10^-1～12μg/mL,9.1×10^-2～7.3μg/mL and 1.63×10^-1～13μg/mL, respectively.Their average recoveries were 97.4%,98.6%,99.1%,101.6%and 99.3%, respectively.The method is simple,rapid,sensitive,accurate,which can provide a basis for quality control of rape honey.更多还原