【作者】 王强； 张津校； 尹靖东； 李凤娜； 杨飞云； 卢媛；

【Author】 Wang-qiang~1,Zhang-jinxiao~1,Yin-jingdong~1, Li-fengna~1,Yang-feiyun~2,Lu-yuan~2 (1.National Key Laboratory of Animal Nutrition,China Agricultural University Beijing 100094;2.Chongqing Academy of Animal Sciences,Chongqing 420460)

【机构】 中国农业大学动物营养国家重点实验室； 重庆市畜牧科学院；

【摘要】 lipin1基因,发现于脂肪肝代谢综合征的老鼠,在老鼠和人的脂肪代谢调节方面都起着重要的作用。猪lipin1基因cDNA从背最长肌中通过反转录PCR克隆得到,编码的氨基酸序列与狗、人和老鼠的同源性分别达到了91%、89%和88%。利用半定量RT-PCR技术测定不同品种猪肌肉中lipin1基因和脂肪代谢相关基因mRNA的表达量。试验数据显示,lipin1基因在脂肪型猪(荣昌猪)肌肉组织中的表达量显著高于瘦肉型猪(PIC猪)(P<0.05)。同时,aP2,C/EBPα,PPARγ和PEPCK基因的表达量在荣昌猪中也显著高于PIC猪(P<0.05),其中FAS基因极其显著(P<0.01)。本研究发现,lipin1基因在猪的肌肉组织中参与脂肪代谢调节的过程,对肌内脂肪沉积起着一定的作用。更多还原

【Abstract】 Lipin1,discovered from the fatty liver dystrophy(fld) mouse,has an important role in regulating the lipid metabolism in the mouse and human.Porcine lipin1 cDNA was cloned from pig longissimus Dorsi(LD) muscle using polymerase chain reaction(PCR) and show 91%,89%and 88%amino acid sequence identity versus the dog,human and mouse lipin1, respectively.The mRNA expression levels of lipin1 and lipocyte-specific genes were determined by semi-quantification RT-PCR in different genotype pigs.The data show that the mRNA expression of lipin1 gene increased in LD muscle of Rongchang(fatty pig) group than PIC (lean pig) group(P<0.05).Meanwhile,aP2,C/EBPα,PPARγand PEPCK expression also increased in Rongchang pigs(P<0.05),and FAS gene expression significantly increased(p<0.01).The study suggest that lipin1 gene expressed in the LD muscle of pigs may have a crucial effect on the lipid metabolism and have an important role in IMF content of fatty pigs.更多还原