【作者】 王林杰； 熊远著； 左波； 徐德全； 任竹青； 雷明刚；

【Author】 Wang-linjie,Xiong-yuanzhu~1,Zuo-bo,Xu-dequan,Ren-zhuqing,Lie-minggang (Key Laboratory of Swine Genetics and Breeding,Ministry of Agriculture;Key Lab of Agriculture Animal Genetics,Breeding and Reproduction,Ministry of Education;College of Animal Science,Huazhong Agricultural University,Wuhan,Hubei,430070,P.R.China)

【机构】 农业部猪遗传育种重点开放实验室&农业动物遗传育种与繁殖教育部重点实验室华中农业大学；

【摘要】 腺苷一磷酸脱氨酶（adenosine monophosphate deaminase,AMPD）是嘌呤代谢过程中的一种重要的酶,在骨骼肌的能量代谢中起着重要作用。本研究分离克隆了猪AMPD1基因及其启动子序列。通过Real-time RT-PCR分析发现AMPD1基因特异在猪骨骼肌中表达,并且在肌肉发育不同阶段的表达趋势在大白和梅山猪中是大致相同的。均是随着肌肉的发育逐渐上调,在180d达到表达量的最大值。并且在背最长肌和股二头肌（Ⅱ型肌纤维,快速糖酵解型）的表达量明显高于比目鱼肌和咬肌（Ⅰ型肌纤维,慢速氧化型）。在其第12外显子上发现一处T/C突变。采用Eco81I-PCR-RFLP方法对234头大白×梅山F₂代资源家系进行性状关联分析,结果表明TT基因型与CC基因型相比具有更高的眼肌面积和眼肌高以及更低的平均背膘厚。这些研究结果表明猪AMPD1基因可能是影响猪生产性状的候选基因,为以后进一步研究其在猪肌肉发育中的功能提供了一定的信息。更多还原

【Abstract】 AMPD1 is the muscle-specific form of the AMPD multigene families in mammals and plays an important role in the purine nucleotide cycle and energy metabolism in skeletal muscle.In this study,we cloned and characterized AMPD1 from porcine muscle.RT-PCR analyses showed that AMPD1 was expressed specifically in skeletal muscle.Expression of AMPD1 was up-regulated during the muscle development and was higher in Yorkshire than in Meishan pigs.AMPD1 gene was expressed at higher levels in longissimus dorsi and bicepsfemoris muscles compared with soleus and masseter muscles in both Yorkshire and Meishan pigs.Moreover,we found that a single nucleotide polymorphism(SNP,T/C⁴²⁶) in exon12 of the AMPD1 gene was significantly associated with loin muscle area trait（P<0.01）,loin muscle height（P<0.01） and average backfat thickness（P<0.05）.This result suggests that the AMPD1 gene might be a candidate gene of meat production trait and provides useful information for further studies on its roles in porcine skeletal muscle.更多还原