【作者】 李明洲； 官久强； 李庆海； 李祥； 帅素容； 李学伟；

【Author】 Li-mingzhou,Guan-yongqiang,Li-qinghai,Li-xiang,Shuai-surong,Li-xuewei~* (College of Animal Science and Technology,Sichuan Agricultural University,Ya’ an 625014)

【机构】 四川农业大学动物科技学院；

【摘要】 动物个体的皮下脂肪厚度(subcutaneous fat thickness,SFT)和肌内脂肪(intramuscularfat,IMF)含量取决于活体内脂肪酸合成与氧化竞争的动态平衡点。利用包含140个与猪脂肪沉积、代谢和肌肉生长密切相关基因的Oligo功能分类基因芯片检测了脂肪型的太湖猪和瘦肉型的长白猪在1,2,3,4和5月龄间背部皮下脂肪中这些基因的动态表达变化,发现长白猪有25个基因在月龄间的表达差异达显著水平(P<0.05),且包含23个基因的"参与脂肪或类固醇代谢的酶和调节蛋白"基因分组在品种间具有极显著意义(P<0.01)。提示两猪种脂肪沉积能力的巨大表型差异可能与这些基因的差异表达规律密切相关。更多还原

【Abstract】 The competitive equilibrium of fatty acid biosynthesis and oxidation in vivo determines porcine sub-cutaneous fat thickness(SFT) and intramuscular fat(IMF) content. Obese and lean-type pig breeds show obvious differences in adipose deposition;however, the molecular mechanism underlying this phenotypic variation remains unclear.We used pathway -focused oligo microarray studies to examine the expression changes of 140 genes associated with meat quality and carcass traits in backfat at five growth stages(1-5 months) of Landrace (a leaner,Western breed) and Taihu pigs(a fatty,indigenous,Chinese breed).Variance analysis (ANOVA) revealed that differences in the expression of 25 genes in Landrace pigs were significant(FDR adjusted permutation,P<0.05) among 5 growth stages.Gene class test(GCT) indicated that a gene-group was very significant between 2 pig breeds across 5 growth stages(P<0.01),which consisted of 23 genes encoding enzymes and regulatory proteins associ-ated with lipid and steroid metabolism.These findings suggest that the distinct differences in fat deposition ability between Landrace and Taihu pigs may closely correlate with the expression changes of these genes.更多还原