【作者】 裴小英； 周东海； 郭定宗； 高红梅； 夏天； 程大池；

【Author】 PEI Xiao-ying,ZHOU Dong-hai~*,GUO Ding-zong,GAO Hong-mei;XIA Tian;CHENG Da-chi (College of veterinary Medicine,Huazhong Agriculture University,Wuhan,Hubei,430070,China)

【机构】 华中农业大学动物医学院；

【摘要】 以体重为(10±0.3)kg,25-28日龄的30头早期断奶仔猪为研究对象,对其中的18头仔猪接种产肠毒素大肠杆菌(ETEC)而建立腹泻模型,免疫组化技术检测蛋白酶激活受体-2(PAR-2)在断奶仔猪胃肠道中的定位,RT-PCR技术检测在健康断奶仔猪和腹泻断奶仔胃肠道中随着腹泻时间变化PAR-2 mRNA的表达变化。免疫组化结果显示PAR-2在早期断奶仔猪整个胃肠道中都有表达,且主要存在于粘膜固有层和免疫细胞,在胃腺和肠腺中有高表达,Real-time RT-PCR结果显示在腹泻仔猪中PAR-2的表达量明显增加,随着腹泻病程的延长,PAR-2表达量显著增加。结果提示PAR-2在断奶仔猪腹泻病理过程有一定的调节作用。更多还原

【Abstract】 Thirty piglets,were 25-28 days old,the average weight were(10±0.3)kg.,eighteen of them were vaccinated enterotoxigenous E.coli(ETEC).Immunohistochemistry was used to detect quantity of PAR2.Real-time RT-PCR technique was used to detect the change of expression of PAR-2 in colon with the course of disease.Immunohistochemical analyses clearly showed abundant PAR-2 in the GI tract of piglet,especially in thelamina propria of mucosae and immunocyte.The result of Real-time RT-PCRshowed that the expression of PAR-2 was increased clearly in stomach、ileum and colon with the time of diarrhea was extended.The results indicated that PAR2 did a role of regulation in diarrheal process of piglet.更多还原