【作者】 范书才； 李虹； 袁率珍； 巢伟； 王少英； 林旭野； 张毓金； 朱山林； 张兵；

【Author】 FAN Shu-cai~1,LI Hong~1,YUAN Shuai-zhen~1,CHAO Wei~2,WANG Shao-ying~2,LIN Xu-ye~2, ZHANG Yi-jin~2,ZHU Shan-lin~2,ZHANG Bing~1 (1.China Institute of Veterinary Drug Control,Beijing 100081;2.Guangdong Winsun Bio-Pharmaceutical CO.,LTD,Guangzhou 511365)

【机构】 中国兽医药品监察所； 广东永顺生物制药有限公司；

【摘要】 从近年来在广东地区流行的鸭肝炎病例中分离到1株病毒（命名为"GD株"）。对分离的病毒进行了鸭胚传代培养。DE₂-DE₆鸭胚毒的ELD₅₀在10^-5.17-10^-6.38/0.2mL之间。鸭胚毒以100 ELD₅₀/0.2mL接种可以致死80%以上的10-11日龄易感鸭胚,接种鸭胚原代肝细胞单层可以产生细胞病变。DE₄代毒对鸭胚的ELD₅₀与对雏鸭的LD₅₀基本一致,回归雏鸭后可以产生与1型鸭肝炎病毒相同的临床症状和解剖病变。鸭肝组织毒对4、8和16日龄雏鸭的LD₅₀分别为10^-6.5/0.5mL、10^-5.38/0.5mL和10^-4.83/0.5ml。病毒对氯仿、乙醚和胰酶不敏感,能够耐受pH3.0酸处理,62℃30分钟不能被完全灭活。病毒核酸类型鉴定为RNA病毒。生物学和理化学特性鉴定结果表明,GD株为强毒鸭肝炎病毒。使用标准1型DHV阳性血清进行的交叉中和试验和交叉被动保护试验表明,GD株病毒在抗原性上与1型鸭肝炎病毒无关,属于一种在我国流行的新型鸭肝炎病毒强毒（暂命名为"CN-DHV"）。更多还原

【Abstract】 A virus strain（designated GD） was isolated from death ducklings with duck hepatitis occured in Guangdong recently. The isolated virus was passed with duck embryos.The virus titers of DE₂～DE₆ passages adapted to duck embryos are from 10^5.17to 10^6.38 ELD₅₀（50%embryos lethal dose） per 0.2 ml.The duck embryos viruses can cause the death of over 80%duck embryos with 10-11 days-old by inocubation with 100 ELD₅₀ per 0.2 ml and the cell-pathogenic effects of primary duck embryos liver cells.The DE₄ virus contains the ELD₅₀ to duck embryos similar to the LD₅₀（50%lethal dose） to ducklings.The intramuscular injection of duck embryos viruses to ducklings can cause the same clinical symptoms and pathological changes caused by the type 1 duck hepatitis virus（DHV-1）.The LD₅₀ of duck liver tissue virus to the 4-,8-and 16-days-old ducklings are 10^-6.5,10^-5.38and 10^-4.83 per 0.5 ml,respectively.The virus is not sensitive to chloroform,diethyl ether and trypsin and possesses acid-resisting and heat-resisting features.The GD strain belongs to RNA virus showed by nueic acid type test.The biological and physicochemical characterization revealed that GD strain is a virulent DHV.The GD strain was determined to be antigenically unrelated to DHV-1 by in vitro cross-neutralization assay and in vivo cross-protection test with standard antiserum against DHV-1.Therefore,GD strain is a new serotype of DHV occurred in China（designated CN-DHV）.更多还原