【作者】 何自福； 虞皓； 罗方芳；

【Author】 He Zifu,Yu Hao,Luo Fangfang (Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

【机构】 广东省农业科学院植物保护研究所；

【摘要】 根据已报道的烟粉虱传双生病毒DNA序列保守区,设计了用于扩增该保守区片段的简并引物,用该引物对广东番茄上似双生病毒病样本进行PCR检测,可以扩增出预期大小的356bp特异片段;利用NCBI中的BLAST程序对该特异片段序列进行比较分析,结果表明,该特异片段是双生病毒DNA片段。因此,可以用该引物来检测广东番茄上烟粉虱传双生病毒。试验结果还显示,广东番茄上烟粉虱传双生病毒该特异片段与已报道的双生病毒及广西番茄上双生病毒间的同源率均小于82%,说明广东番茄上烟粉虱传双生病毒与它们可能存在较大的差异。更多还原

【Abstract】 According to the conserved regions of the reported nucleotide sequences of whitefly-transmitted geminiviruses(WTG),a pair of degenerate primer was designed to anneal to the conserved sequence.The tomato samples infected geminivirus - like from Guangdong were detected by PCR.The results showed that a 356bp specific fragment was amplified from the samples.The specific fragment was cloned and sequenced,and the sequence was compared with all nucleotide sequences in Genbank by Blast of NCBI.The result showed that the fragment belonged to Geminiviridae DNA.So the degenerate primers may be used to detect the WTG from tomato in Guangdong.Moreover,both of the homology of the fragment between WTG from tomato in Guangdong and the reported WTG in the world and WTG from tomato in Guangxi were under 82%.These results implied that the WTG from tomato in Guangdong differed from the above - mentioned WTG.更多还原