【作者】 潘淑勇； 谢双大； 林壁润； 江学斌； 沈会芳； 周小毛； 刘坚真；

【Author】 Pan Shuyong~(1,2),Xie Shuangda~1,Lin Birun~1,Jiang Xuebing~1, Shen Huifang~1,Liu Jianzhen~2 (1.Plant Protection Research Institute,Guangdong Academy of Agriculture Science,Guangzhou 510640,China; 2.Food Science College,South China Agriculture University,Guangzhou 510642,China)

【机构】 广东省农业科学研究院植物保护研究所； 华南农业大学食品学院；

【摘要】 本研究建立了以硅胶板层析法为基础,以枯草杆菌作为指示菌的生物自显影法作为农抗2507活性组成的常规定性检测方法:用50mg/ml农抗2507粗提物点样0.5μl,用三元溶剂乙酸乙酯:异丙醇:正己烷=71.5:16.8:11.7展开,贴板10min,37℃培养10h即可得到清晰的抑菌斑色谱图。应用该方法可将2507粗提取物分离得到10个活性组分。更多还原

【Abstract】 Agri - antibiotic 2507 is a new kind of agri - antibiotic against Oomyces.We have established a qualitative analysis to determin the component of agri - an,tibiotic 2507.During this determination the silica gel thin - layer chromatography and the biological development are used.The 50mg/ml alcohol solution of 2507 is used for ditermination.The optimum quantity of sample application is 0.5μl.The developing solvent is a kind of ternary organic solvent that is composed of ethyl acetate, isopropyl alcohol and hexane in the ratio of 71.5 to 16.8 to 11.7.The diffusion time is 10min. The indicator organism is Bacillus subtilis.The culture time is 10h under the tempreture of 371.This qualitative analysis can separate agri - antibiotic 2507 into 10 different active components that were named D1,D2,A2,A1,B1,B2,B3,D1,D2,D3 respectivly,this method proved to be simple and convenient and can be used to identify and distinguish the active substances of agri - antibiotic 2507.更多还原