【作者】 马元伟； 马康； 王守现； 赵爽； 王兰青； 刘宇； 许峰；

【Author】 MA Yuan-Wei;MA Kang;WANG Shou-xian;ZHAO Shuang;WANG Lan-Qin;LIU Yu;XU Feng;Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing Engineering Research Center for Edible Mushroom;Key Laboratory of Urban Agriculture(North),Ministry of Agriculture;

【机构】 北京市农林科学院植物保护环境保护研究所北京市食用菌工程技术研究中心； 农业部都市农业(北方)重点实验室；

【摘要】 从北京房山地区杏鲍菇工厂化生产车间感染软腐病的刺芹侧耳子实体中分离得到四株革兰氏阴性兼性厌氧菌,各菌株16SrRNA和rpoB基因序列测定结果表明分离到的菌株为泛菌属Pantoea。基于atpD,gyrB,,infB和rpoB基因的多位点序列分析表明这些菌株与分散泛菌Pantoea dispersa遗传关系最近,并可能组成一个新种,生理生化性质测定结果表明本研究分离到的菌株与分散泛菌P.dispersa存在明显区别,DNA—DNA同源性杂交试验结果进一步的证实了分离到的菌株为泛菌属新种,命名为北京泛菌(LMG 27579~T=KCTC 3240~T)。柯赫氏法则证明了北京泛菌是北京地区发生的杏鲍菇软腐病病原菌。更多还原

【Abstract】 Four Gram-negative-staining,facultatively anaerobic bacterial isolates were obtained from the fruiting bodies of the edible mushroom Pleurotus eryngii showing symptoms of soft rot disease in Beijing,China.Partial16 S rRNA gene sequencing,together with partial rpoB sequencing,placed these isolates in the genus Pantoea.Multilocus sequence analysis(MLSA) based on the partial sequences of atpD,gyrB,infB,and rpoB revealed Pantoea dispersa as their closest phylogenetic relatives and indicated that these isolates constituted a possible novel species.DNA-DNA hybridization studies confirmed the classification of the new isolates as a novel species and phenotypic tests allowed for differentiation from the closest phylogenetic neighbours.Pantoea beijingensis sp.nov.[type strain LMG 27579~T= KCTC 32406~T]is proposed.Koch’s postulates proved that Pantoea beijingensis sp.nov is the pathogenic bacteria of soft rot disease on Pleurotus eryngii occurring in Beijing,China.更多还原