【作者】 陈文强； 邓百万； 殷书学； 彭浩； 解修超；

【Author】 CHEN Wen-qiang~(1,2),DENG Bai-wan~(1,2),YIN Shu-xue~3,PENG Hao~(1,2),XIE Xiu-chao~(1,2) (1.School of Biological Science and Engineering,Shaanxi University of Technology,2.Shaanxi Engineering Research Center of Edible and Medicated Fungi,Hanzhong 723000,3.Liuba Edible Fungi Experiment Station,Liuba 724100 Shaanxi)

【机构】 陕西理工学院生物科学与工程学院； 陕西省食药用工程技术研究中心； 陕西留坝真菌研究所；

【摘要】 采用摇瓶培养法对猪苓胞内多糖的发酵条件进行了优化。结果表明,液体发酵生产猪苓胞内多糖的最适碳源是蔗糖,最适氮源是黄豆粉,优化培养基组成是:马铃薯10.0%,蔗糖4.0%,黄豆粉0.3%,KH₂PO₄0.2%,MgSO₄·7H₂O 0.15%;液体发酵生产胞内多糖的优化条件为;初始pH5.2～5.6,振荡速度180～200 r/min,培养温度28℃,培养时间7d。更多还原

【Abstract】 This paper explore that suitable fermentative conditions,which affect the extraction rate of intracellular polysaccharide of Polyporus umbellatus.Experimental results indicated that,the best carbon sources is saccharose,the best nitrogen sources is soybean powder,the optimum composition of a fermentative medium is as following:potato 10.0%,saccharose 4.0%,soybean powder 0.3%,KH₂PO₄ 0.2%, MgSO₄-7H₂O 0.15%.The highest yield of intracellular polysaccharide was obtained after fermentation for 7 days on the conditions that the shaker rotation speed is atl80～200r/min,pH5.2～5.6,the temperature is at 28℃.更多还原