【作者】 王立安； 李洁； 张树斌； 赵俊霞；

【Author】 WANG LiAn~1 LI Jie~1 ZHANG ShuBin~1 ZHAO JunXia~2 (1 The Life Science College,Hebei Normal University,Shijiazhuang 050016,China;2 The Basic Medical College,Hebei Medical University,Shijiazhuang 050017,China)

【机构】 河北师范大学生命科学学院； 河北医科大学基础医学院；

【摘要】 采用石油醚(PE)、乙酸乙酯(EA)、乙醇(Eth)三种有机溶剂分别萃取黄乳牛肝菌(Suillus luteus)子实体干粉,得到三种有机相提取物,得率分别为3.9%、3.27%和4.23%。随后,对萃取后的干粉采用80℃的热水浸提12 h,浸提液经两倍体积95%乙醇过夜沉淀,得子实体多糖,得率为18.69%。对三种有机相提取物中的总酚、粗三萜和总黄酮的含量分析表明,三种有机相提取物中均含这三种抗氧化成分,但含量不同。其中,乙酸乙酯提取物的总酚含量最高,为6.4%,明显高于石油醚(4.62%)和乙醇提取物(4.80%);后两者差异不显著(p<0.05)。石油醚、乙酸乙酯提取物的粗三萜含量分别为20.35%和19.18%,明显高于乙醇提取物(8.59%);乙酸乙酯提取物的总黄酮含量最高,为4.92%,明显高于石油醚(1.7%)乙醇提取物(0.43%)。更多还原

【Abstract】 The extracts from the fruitbody of Suillus luteus were obtained by using different pola organic solvent,including petroleum ether（PE）,ethylacetate（EA） and ethanol（Eth）. Then polysaccharides from the mushrooms were prepared by using hot water extraction and ethanol precipitation.The yield of extracts was 3.9%,3.27%,4.23%and 18.69%, respectively. （1） The contents of antioxidant component in the extracts prepared by organic solvent, including polyphenols,triterpenes and flavonoids were measured.As a result,there were different polyphenols,triterpenes and flavonoids among every organic extracts,including PE-soluble portion,EA-soluble portion and Eth-soluble portion.The content of polyphenols involved in EA-soluble portion（6.4%） was more than that in PE-soluble portion（4.62%） and Eth-soluble portion（4.8%）.The contents of triterpenes existed in PE-soluble portion（20.35%） and EA-soluble portion（19.18%） were more than that in Eth-soluble portion（8.59%）.The content of flavonoids in EA-soluble portion was（4.92%） the most,and remarkably more than that in PE-soluble portion（1.7%） and Eth-soluble portion（0.43%）. （2） The antioxidant assays of every extracts in vitro were carried out by four methods, including DPPH radical-scavenging method,inhibition of lipid peroxidation, determination of reducing power and metal ion chelating activity.The results showed that each extracts exhibited different antioxidant activity in vitro. The IC50 value of DPPH radical-scavenging assays of the three kinds of organic extracts was 0.00015 mg/mL（PE）,0.18 mg/mL（EA） and 0.26 mg/mL（Eth）,respectively,and lower than that of the polysaccharide（6.74 mg/mL）.The organic extracts were more effective than the polysaccharides（46.82 mg/mL） in inhibition of lipid peroxidation.The IC50 value of three organic extracts was 0.04 mg/mL,0.08 mg/mL,0.14 mg/mL,respectively.The EA-soluble portion had the highest reducing power with OD value of 0.479 at 10 mg/mL.The OD value of the PE-soluble portion,Eth-soluble portion and polysaccharides was 0.342,0.26, 0.205,respectively.In Fe²⁺ chelating activity assay,the Eth-soluble portion and polysaccharides had better chelating activity than other extracts,and the IC50 value was 2.78 mg/mLand 1.44 mg/mL,respectively. （3） Aged mice caused by D-galactose were perfused by EA-soluble portion of Suillus luteus using ascorbic acid（Vc） as control.The results showed that the EA-soluble portion of Suillus luteus could increase the activities of SOD and GSH-px and decrease the content of MDA in serum and liver of mice.The effect of the extracts was equal to or better than that of ascorbic acid. Under the treatment with 400 mg/（kg·d） dose of EA-soluble portion of Suillus luteus,the activity of SOD would be increased to 467.7 U/mL in serum and 41.02 U/mL in liver of mice.Also,the activity of GSH-px would be increased to 385.56 unit in serum and 3.15 unit in liver.Compared to normal group and aged group,the activities of SOD and GSH-px were significantly increased.The content of MDA in serum and liver were significantly decreased to 3.65 nmol/mL and 0.33 nmol/mg prot,respectively.These suggested that the EA-soluble portion of Suillus luteus could increase the antioxidant ability of aged mice in vivo.更多还原