【作者】 韩玉梅； 金莹； 李安娜； 孔怡； 丁爱云；

【Author】 HAN Yumei~1 JIN Yiing~(2,3) LI Anna~1 KONG Yi~4 DING Aiyun~(1*) (1.Department of Plant Protection,Shandong Agricultural University,Tai’an 271018;2.Huangdao Entry-Exit Inspection and Quarantine Bureau Qingdao 266000 3.College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083;4.Research Insstitute of Edible,Shandong Tai’an Academy of Agricultural Sciences,Tai’an 271000)

【机构】 山东农业大学植物保护学院； 山东黄岛检验检疫局； 北京林业大学生物科学与技术学院； 山东泰安市农科院；

【摘要】 本文报道了采自山东省泰山的泰山蛹虫草,采用组织分离法分离获得泰山虫草无性型菌株,并在大米培养基上培养出子实体。用泰山虫草分生孢子悬浮液注射活体黄粉虫,提取被侵染的黄粉虫体液,并测定不同侵染时间内虫体液中酶活性物质的动态的变化。结果表明,泰山虫草分生孢子能成功的侵染黄粉虫并长出子实体;被侵染的黄粉虫体液中活性物质含量的测定表明,从接种第2d到第4d即可导致其虫体内活性物质发生不同程度的变化,说明黄粉虫具有独特的防御病原微生物侵染的机制。更多还原

【Abstract】 In this paper Taishan cordyceps conidiophore suspensoid was injected into living Tenebrio molitor L..Taishan cordyceps sp.can infected Tenebrio molitor L.successfully and grew out Sporophore.Then the body fluid of the T.molitor was distilled and some of the enzyme activities such as SOD,POD,CAT in it were determined to be changed in different time.The result showed there were some special mechanisms in the T.molitor to defense the infection of the pathogen.更多还原