【作者】 钟韩； 甘莉霞； 章卫民； 潘清灵； 李浩华； 陶美华； 王磊； 谭毓治； 刘艳萍；

【Author】 ZHONG Han1,GAN Lixia1,ZHANG Weimin1,PAN Qingling1,LI Haohua1,TAO Meihua1,WANG Lei1,TAN Yuzhi2,LIU Yanping3 (1 Guangdong Institute of Microbiology,Guangzhou,China;2Guangdong College of Pharmacy, Guangzhou,China;3Guangdong Jin Chongcao Biological develepment Co.Ltd.,Guangzhou,China)

【机构】 广东省微生物研究所； 广东药学院； 广东金虫草生物开发有限公司；

【摘要】 目的测定野生戴氏虫草的多糖、腺苷、甘露醇等主活化学成分,观察戴氏虫草水提液对小鼠免疫调节的作用,同时测定小鼠经口LD50;方法采用苯酚硫酸法测定虫草多糖,HPLC法测定腺苷和和甘露醇;用NIH小鼠进行碳廓清试验、免疫器官重量和迟发型超敏反应试验;结果野生戴氏虫草的多糖、腺苷及甘露醇含量分别为36.20mg/、0.27mg/g、80.20mg/g,戴氏虫草水提液能提高非特异性免疫功能,提高受环磷酰胺抑制的小鼠免疫器官重量和细胞免疫功能;戴氏虫草水提液小鼠经口LD50>69.42g/kg。更多还原

【Abstract】 Objective To analyze the main active components including polysaccharide, adenosine and mannitol of wild Cordyceps taii,observe its immune regulation in mice and additionally assay the oral LD50.Methods The phenol-sulfuric acid method and HPLC were used to detect the components of C.taii.The carbon clearance test,the weight determination of the immune organs and the delayed-type hypersensitivity test(DTH) in NIH mice were used to study the immune regulation of C.taii.Results The contents of polysaccharide, adenosine and mannitol of wild Cordyceps taii are 36.20mg/、0.27mg/g、80.20mg/g respectively and its water extract can increase nonspecific immunity,the weight of the immune organs inhibited by cyclophosphamide and cellular immune function in mice.The oral LD50 of C.taii water extract>69.42g/kg.更多还原