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野生戴氏虫草活性成分的测定及其对免疫调节作用的研究
Studies on Active Components of Cordyceps taii and its Immune Regulation in Mice
【作者】 钟韩; 甘莉霞; 章卫民; 潘清灵; 李浩华; 陶美华; 王磊; 谭毓治; 刘艳萍;
【Author】 ZHONG Han1,GAN Lixia1,ZHANG Weimin1,PAN Qingling1,LI Haohua1,TAO Meihua1,WANG Lei1,TAN Yuzhi2,LIU Yanping3 (1 Guangdong Institute of Microbiology,Guangzhou,China;2Guangdong College of Pharmacy, Guangzhou,China;3Guangdong Jin Chongcao Biological develepment Co.Ltd.,Guangzhou,China)
【机构】 广东省微生物研究所; 广东药学院; 广东金虫草生物开发有限公司;
【摘要】 目的测定野生戴氏虫草的多糖、腺苷、甘露醇等主活化学成分,观察戴氏虫草水提液对小鼠免疫调节的作用,同时测定小鼠经口LD50;方法采用苯酚硫酸法测定虫草多糖,HPLC法测定腺苷和和甘露醇;用NIH小鼠进行碳廓清试验、免疫器官重量和迟发型超敏反应试验;结果野生戴氏虫草的多糖、腺苷及甘露醇含量分别为36.20mg/、0.27mg/g、80.20mg/g,戴氏虫草水提液能提高非特异性免疫功能,提高受环磷酰胺抑制的小鼠免疫器官重量和细胞免疫功能;戴氏虫草水提液小鼠经口LD50>69.42g/kg。
【Abstract】 Objective To analyze the main active components including polysaccharide, adenosine and mannitol of wild Cordyceps taii,observe its immune regulation in mice and additionally assay the oral LD50.Methods The phenol-sulfuric acid method and HPLC were used to detect the components of C.taii.The carbon clearance test,the weight determination of the immune organs and the delayed-type hypersensitivity test(DTH) in NIH mice were used to study the immune regulation of C.taii.Results The contents of polysaccharide, adenosine and mannitol of wild Cordyceps taii are 36.20mg/、0.27mg/g、80.20mg/g respectively and its water extract can increase nonspecific immunity,the weight of the immune organs inhibited by cyclophosphamide and cellular immune function in mice.The oral LD50 of C.taii water extract>69.42g/kg.
【Key words】 Cordyceps taii; polysaccharide; adenosine; mannitol; carbon clearance; immune regulation;
- 【会议录名称】 2008全国药用真菌学术研讨会论文集
- 【会议名称】2008全国药用真菌学术研讨会
- 【会议时间】2008-10
- 【会议地点】中国江西南昌
- 【分类号】R284
- 【主办单位】中国菌物学会药用真菌专业委员会、中国药学会药用真菌专业组