【作者】 龚金炎； 葛青； 黄俊； 毛建卫； 张英；

【Author】 Jinyan Gong;Qing Ge;Jun Huang;Jianwei Mao;Ying Zhang;Zhejiang provincial Key Lab for Chem & Bio Processing Technology of Farm produces;School of Biological and Chemical Engineering,Zhejiang University of Science and Technology;College of Biosystem Engineering and Food Science,Zhejiang University;Shaoxing Testing Institute of Quality Technical Supervision;

【机构】 浙江省农产品化学与生物加工技术重点实验室； 浙江科技学院生物与化学工程学院； 浙江大学生物系统工程与食品科学学院； 绍兴市质量技术监督检测院；

【摘要】 建立一种反相高效液相色谱（RP-HPLC）法测定竹茹提取物及其不同部位的腺苷含量的方法。采用RP-HPLC法;Luna C₁₈ODS柱（4.6 mm×250mm,5μm）,乙腈-0.01 mol/L磷酸缓冲溶液（pH=6.5）;体积流量:1.0 mL/min;进样量:10μL;柱温:40℃;检测波长:257 nm。结果采用RP-HPLC法竹茹提取物及其不同部位的腺苷含量,在0.121.124μg/mL范围内线性关系良好（R²=0.9999）,平均回收率为98.1-99.1%,RSD为1.98-3.11%,检测限为40 ng/mL。本方法简便、灵敏、准确,可普遍应用于竹茹提取物的质量研究和品质控制。更多还原

【Abstract】 To develop a method for determination of adenosine in extract of bamboo shavings by RP-HPLC.RP-HPLC method was used with Luna C₁₈ODS（4.6 ×250mm,5 μm） column.The mobile phase was acetonitrile-0.01 mol/L phosphate buffer solution（pH =6.5） with 1.0 mL/min flow rate;The column temperature was 40 ℃;The detection wavelength was set at 257 nm.RP-HPLC method was used for the determination adenosine;The calibration curve of adenosine was linear under the content 0.12-124 μg/mL;The linearity the average recovery was from 98.1-99.1%,.RSD was 1.98-3.11%,limite of detection is 40 ng/mL.Conclusion The method is simple,sensitive and accurate.The method can be adapted to control the quality of extract of bamboo shavings.更多还原