【作者】 李栋栋； 罗自生；

【Author】 Dongdong Li;Zisheng Luo;Department of Food Science and Nutrition,Zhejiang University;

【机构】 浙江大学食品科学与营养系；

【摘要】 草莓富含花青素,进一步了解草莓成熟过程中花青素合成机理对于提高草莓营养价值具有重要作用。本研究通过比较对照、脱落酸(ABA)、去甲二氢愈创木酸(ABA合成抑制剂)三个处理组间三个不同阶段(第O、5、8天)的转录组de novo拼接数据,发现了花青素合成途径上编码蛋白酶的共116个unigenes,其中有39个unigenes在草莓整个生长过程中显著性差异表达,包括10个编码4-香豆酸:辅酶A连接酶unigenes,2个查尔酮合酶unigenes,2个查尔酮异构酶unigenes,3个黄烷酮-3-羟化酶unigenes,10个二氢黄烷酮醇-4-还原酶unigenes,1个花青素合成酶unigenes以及11个UDP葡萄糖-类黄酮-3’-O-葡糖基转移酶unigenes。第5天时,ABA显著上调了34个unigenes的表达量,其中20个unigenes至第8天仍受到显著上调。此外,结果还显示比对到的4个编码肉桂酸-4-羟化酶unigenes在草莓成熟过程中低表达,并且不受ABA调控,可见肉桂酸-4-羟化酶不是花青素合成途径上的限速酶。研究不仅深入探索了在草莓成熟过程中ABA对花青素生物合成途径上各类编码蛋白酶基因的调控作用,还丰富了草莓花青素合成方向的生物学信息。更多还原

【Abstract】 Strawberry fruit is rich in anthocyanins,and better understanding of the process of anthocyanins biosynthesis during strawberry ripening is substantial to elevate its nutritional value.In present study,data of de novo transcriptome in strawberry fruit(Fragaria ananassa Duch.) was compared between control,Abscisic acid(ABA) and nordihydroguaiaretic acid(NDGA,an ABA biosynthesis blocker) treatments at 3different stages(day 0,5 and 8 after treatment).Analysis identified 116 unigenes which encode enzymes on anthocyanins biosynthesis pathway during strawberry ripening.Among these unigenes,39 were differential expressed unigenes,including 10 unigenes encoding 4-coumarate:CoA ligase,2 unigenes encoding chalcone synthase,2 unigenes encoding chalcone isomerase,3 unigenes encoding flavanone 3-hydroxylase,10 unigenes encoding dihydroflavonol 4-reductase,one unigene encoding anthocyanidin synthase and 11 unigenes encoding UDP-glucose:flavonoid glucosyltransferase.On day 5,expression of 34 unigenes were significantly enhanced with ABA application,in which,20 unigenes were still up-regulated by ABA on day8.Results also showed that low expression was found in 4 unigenes encoding cinnamate-4-hydroxylase(C4H).Additionally,expression of these 4 unigenes was not regulated by ABA,indicating that C4 H was not a rate-limiting enzyme on anthocyanins biosynthesis pathway in strawberry fruit.This study not only investigates the role of ABA plays on genes expression of anthocyanins biosynthesis related enzymes during strawberry ripening,also provides abundant biology information pertaining to anthocyanins biosynthesis.更多还原