【作者】 白会钗； 缪铭； 江波；

【Author】 Huichai Bai Ming Miao Bo Jiang (State Key Laboratory of Food Science and Technology,Jiangnan University,Jiangsu China 214122)

【机构】 江南大学食品科学与技术国家重点实验室；

【摘要】 乳糖酸是一种具有多种生物学功能的先进果酸,具有多种生物学功能,可以作为功能性组分应用在多种领域。Pseudomonas fluorescenceSK17.001静息细胞可乳糖生成乳糖酸。相较于和一般的转化方法,超高压作用于静息细胞后,改变细胞膜的通透性和结构,使得乳糖酸的转化率得到极大的提升。不同的超高压条件如压力(100-400MPa),温度(20-50℃),pH(3.0-8.0)和作用时间(5-15min)等对乳糖酸的转化率有着很大的影响。本实验通过通过优化超高压作用的参数找到最佳的作用条件,200MPa,10min,温度为30℃,pH5.0时,乳糖酸的转化率比对照提高2-4倍。本研究的结果表明了超高压作为一种经济适用的技术手段可以更快速高效的用于乳糖酸的生产。更多还原

【Abstract】 Lactobionic acid(LA)is a functional organic acid,may have commercial potential as a source of novel functional component or ingredient in a variety of fields.LA was subjected to lactose oxidation assisted with high hydrostatic pressure(HHP)using resting cells of Pseudomonas fluorescence SK17.001.Compared with the conventional enzymatic method for LA(without HHP),HHP was much faster and thus more efficient.The effect of high hydrostatic pressure(100 to 400 MPa)at different temperature(from 20-50℃) on the production of LA by pseudomonas fluorescence SK17.001 was investigated in 50 mM buffers(pH 3.0-8.0),the results implied under pH5.0,200MPa for 10min,the production of LA is almost 2-4times than control.Results from this study indicated that HHP could be a potentially useful and economical technique for a faster and more efficient method for production of LA.更多还原