【作者】 赵轶； 孟庆恒；

【Author】 ZHAO Yi,MENG Qing-heng(College of Life Science,Tianjin Normal University,Tianjin 300387,China)

【机构】 天津师范大学生命科学学院；

【摘要】 本研究从铬矿渣堆放区的土壤环境中获得了对Cr(VI)有抗性和去除能力的好氧性土著细菌Cr-09(Bacillus sp),研究结果表明,该菌能在有氧条件下将菌体及环境中的Cr(Ⅵ)还原为Cr(Ⅲ),并以胞内富集Cr(Ⅲ)的方式实现除铬作用。抗氧化保护酶系的活性和电泳分析结果表明,在Cr(Ⅵ)胁迫条件下,保护酶系中CAT、SOD、POD可通过协同作用对菌体进行保护,使得目标菌将Cr(Ⅵ)还原为Cr(Ⅲ)的作用能够正常进行。更多还原

【Abstract】 Aerobic autochthonous bacterium Cr-09(Bacillus sp)was isolated from chromium polluted soil,which was proved Chromium(Ⅵ) resistance.The results of the experiment showed that,Cr-09 strain could accumulate and reduce Chromium(Ⅵ) into Cr(Ⅲ) intracellular and extracellular environments in aerobic conditions.Activities of antioxidant enzyme and their isozyme electrophoresis were also assayed in the research.The results showed that the antioxidase system was activated when stimulated by the stress of Chromium(Ⅵ) and ensuring them not only surviving under the toxic condition,but also provide a protection to the cells through the coordinative action within CAT,SOD and POD which led to the bacterial cells enable to reduce Cr(Ⅵ) into Cr(Ⅲ) effectively under the stress environment of Chromium(Ⅵ).更多还原