【作者】 高扬； 夏军； 汪亚峰； 毛亮； 周培；

【Author】 GAO Yang1,2,XIA Jun1*,WANG Ya-feng4,MAO Liang3,ZHOU Pei3(1.Key Laboratory of Water Cycle and Related Land Surface Processes,Institute of Geographic Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing 100101,China;2.College of Global Change and Earth System Science,Beijing Normal University,Beijing 100078,China;3.School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;4.State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,CAS,Beijing 100085,China)

【机构】 陆地水循环及地表过程重点实验室,中国科学院地理科学与资源研究所； 全球变化与全球系统科学研究院,北京师范大学； 城市与区域生态国家重点实验室,中国科学院生态环境研究中心； 农业与生物学院,上海交通大学；

【摘要】 由于土壤酶活性可稳定、敏感地反映重金属对土壤的污染程度,因此,本文通过研究Cd、Pb单一及复合污染对土壤酶(脱氢酶、磷酸酶、脲酶、过氧化氢酶、淀粉酶和蔗糖酶)活性的影响,以选取合适的土壤酶作为Cd、Pb污染的生物标记物,为建立土壤Cd、Pb污染的健康修复基准提供科学依据。研究结果表明:不同的土壤酶活性对Cd、Pb的敏感性不同,并且酶活性随土壤重金属浓度增加表现为叠加效应或拮抗效应。Cd、Pb单一污染及复合污染对脲酶活性抑制作用显著,复合污染下脲酶活性表现为叠加效应,因此选择脲酶作为土壤Cd、Pb污染的生物标记物。利用半数生态剂量模型研究发现Cd、Pb对50%脲酶活性抑制的毒性阈值分别为2 273和2 703,并通过脲酶活性与土壤重金属浓度的相关性分析,得出Cd污染土壤的健康修复基准值为1.33 mg Cd.kg-1土,Pb污染土壤的健康修复基准值为106 mg Pb.kg-1土。更多还原

【Abstract】 Due to that soil enzyme activity can stably and sensitively reflect the degree of heavy metal contamination,therefore,the aim of this study is to research on the change of soil enzymes(dehydrogenase,phosphatase,urease,catalase,amylase and sucrase) activities under single Cd or Pb pollution and combined pollution,in order to select the appropriate soil enzymes as a biomarker of Cd and Pb pollution,then providing scientific basis for the establishment of soil health restoration on Cd and Pb pollution.The results showed that the sensitivity of different soil enzyme activities to Cd and Pb were different,and soil enzyme activity exhibited additive effect or antagonistic effect with increasing heavy metal concentration.The effects of single Cd or Pb pollution and combined pollution on urease activity inhibition were significant,and urease activity under combined pollution exhibited additive effect.Thus,urease was selected as a biomarker for Cd and Pb polluted soil.Through 50% ecological dose model research,we found that the 50% ecological dose toxic threshold of urease activity inhibition under Cd and Pb pollution reached 2 273 and 2 703,respectively,and then through the correlation analysis of soil urease activity and heavy metals concentration,we confirmed that the baseline value for the health remediation of Cd contaminated soil was 1.33 mg Cd.kg-1.soil and the baseline value for the health remediation of Pb contaminated soil was 106 mg Pb·kg-1 soil.更多还原