【作者】 彭宁； 黄雪松；

【Author】 PENG Ning,HUANG Xue-song (Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China)

【机构】 暨南大学食品科学与工程系；

【摘要】 呋喃是一种广泛存在于热加工食品中的潜在痕量致癌物质,为了实现方便准确检测食品中的痕量呋喃,本实验以2-甲基呋喃作为模拟模板,甲基丙烯酸作为功能单体,甲基丙烯酸乙二醇酯作为交联剂,采用非共价法合成分子印迹聚合物(MIP)。以该MIP作为固相萃取吸附物质用于富集咖啡中的呋喃,然后对影响固相萃取的各个步骤进行优化,确定最佳条件,结合该条件利用高效液相色谱对咖啡中呋喃进行检测。结果表明呋喃标准溶液的回收率达到80.2%,而咖啡样品加标回收率在62.7%左右,该实验为进一步实现呋喃的方便准确检测奠定基础。更多还原

【Abstract】 Furan was classified to be "possibly carcinogenic to humans"and naturally occurred in many heat treated foods at very low levels.In order to find a convenient and accurate way for the determination of furan,molecular imprinted polymer(MIP) has been synthesized and used as a sorbent to enrich the trace furan to a high level at which could be detected by high performance liquid chromatography(HPLC). The MIP was prepared following a non-covalent protocol with 2-methyl furan as dummy template,methyl acrylic acid(MAA) as functional monomer and ethylene glycol dimethacrylate(EGDMA) as cross-linker.Then,various parameters affecting the extraction efficiency of the solid-phase extraction(MIPSE) have been evaluated to optimize the selective preconcentration of the furan from coffee.The characteristics of the MISPE method were valided by HPLC.The results showed that recoveries of furan standard solution reached nearly 80%and up to 60% from instant coffee sample could be obtained after SPE.It was demonstrated that this MISPE-HPLC method could be applied to direct preconcentration and determination of furan in coffee.更多还原