【作者】 张清丽； 徐皎云； 吴佳娜； 赵谋明；

【Author】 ZHANG Qing-li,XU Jiao-yun,WU Jia-na,ZHAO Mou-ming (College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China)

【机构】 华南理工大学轻工与食品学院；

【摘要】 从酪蛋白水解物中分离纯化和鉴定了一种具有促进乳酸菌生长的活性肽。酪盶酸钠经过木瓜蛋白酶水解后,得到酪蛋白水解物。通过膜分离,大孔树脂,凝胶色谱和高效液相色谱对酪蛋白分解物进行分离纯化。在乳酸菌培养基中添加酪蛋白水解物或各个分离组分,检测不同添加物对乳酸菌的增殖作用。最后通过LC-MS/MS和氨基酸分析对分离纯化后的活性肽进行结构鉴定。结果表明,从酪蛋白水解物中分离得到了能够使乳酸菌增值速度提高将近一倍的六肽,结构鉴定其为Asp-Ile-Pro-Asn-Pro-Ile。本研究得到了能明显促进乳酸菌生长的活性肽,其不仅可以作为强化营养添加剂,而且可以应用于酸乳生产中提高生产效率。更多还原

【Abstract】 A hexapeptide with growth-stimulating activity for lactic acid bacteria(LAB) was purified and characterized from casein hydrolysate.The casein hydrolysate treated with papain was sequentially fractionated by ultrafiltration,macroporous adsorption resin chromatography,gel filtration chromatography and high-performance liquid chromatography.The growth-stimulating activities of hydrolysate and its extracts were testing by the growth performance of LAB in MRS broth medium supplemented with hydrolysate or its extracts.The purified peptide was characterized using LC-MS/MS with electrospray ionization mass spectrometry.A purified hexapeptide with significant growth-stimulating activity for LAB was identified as Asp-Ile-Pro-Asn-Pro-Ile.In this work,a peptide with strong growth-stimulating activity for LAB was obtained,which showed that bioactive peptides from casein could not only serve as a nutrition fortification substances,but also play an important role in the yoghurt manufacture industry for enhancing production efficiency.更多还原