【作者】 魏海柳； 穆燕； 姜锐； 邱俊； 李琳； 张喜梅； 胡松青；

【Author】 WEI Hai-Liu,MU Yan,JIANG Rui,QIU Jun,LI Lin,ZHANG Xi-Mei,HU Song-qing (College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China)

【机构】 华南理工大学轻工与食品学院；

【摘要】 本文研究了白附子提取物对酪氨酸酶活性的影响。通过比较不同溶剂提取物对酪氨酸酶活性的影响,确定出用20%乙醇水-溶液从白附子中提取的活性成分对酪氨酸酶的抑制活性最高,抑制率可达到96%。同时对超声辅助提取法与恒温水浴振荡法进行比较,结果表明超声辅助提取得到的白附子粗提液对酪氨酸酶的抑制活性明显优于同等条件下未施加超声场所提取的。在此基础上,以抑制率为因变量,粗提物浓度为自变量,采用线性回归的方式确定出白附子粗提液对酪氨酸酶的IC₅₀=74.18 mg/mL。更多还原

【Abstract】 Inhibitory effect of the extract from Giant Typhonium Rhizome（GTR） on tyrosinase was investigated.It was identified that crude extract from GTR with 20%ethanol-water solvent had a peak inhibitory rate to tyrosinase among all kinds of studied solvent.As high as 96%inhibitory rate can be obtained.Results showed that extract by ultrasound-extraction had better inhibitory effect to tyrosinase than that by shaking extraction.Based on the dependence of the inhibition rate on the concentration of extract,the IC₅₀ of GTR crude extract to tyrosinase was calculated by linear regression equation, with a value of 74.18 mg/rnL.更多还原