【作者】 梁文杰； 谭晓风； 王立新； 李秀根； 张琳；

【Author】 Liang Wen-jie1,2, Tan Xiao-feng1, Wang Li-xin2,Li Xiu-gen3,Zhang Lin1 (1The Key Lab of Non-Wood Forest Product of Forestry Ministry, Central South University of Forestry and Technology,Changsha Hunan 410004 China, 2Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang 325006 China, 3Zhengzhou Fruit Institute, Chinese Academy of Agriculture Sciences , Zhengzhou, Henan 45009 China)

【机构】 中南林业科技大学经济林育种与栽培国家林业局重点实验室； 温州科技职业学院； 中国农业科学院郑州果树研究所；

【摘要】 以砂梨品种云南宝珠叶片为材料,设计特异引物,从基因组中直接扩增出了梨自交不亲和新基因S42-RNase基因全长序列;并以云南宝珠的雌蕊为材料提取mRNA,通过采用RACE技术,获得了1个844bp梨S基因的cDNA目的片段,经克隆后测序,证实该片段是梨S42-RNase的全长cDNA序列。该基因的编码框由678个碱基组成,共编码226个氨基酸,和已克隆的梨S-RNase基因的cDNA序列的相似性为62%-74%。通过和克隆的S42-RNaseDNA序列的比对分析表明,S42-RNaseDNA序列中包含2个外显子和一个内含子,内含子全长335bp,符合GT-AG规律。更多还原

【Abstract】 A 1141bp sequence of S-RNase gene was isolated by PCR from and A 844bp sequence of S-RNase gene was isolated by rapid amplification of cDNA ends Pyrus pyrifolia Yunanbaozhu. Sequencing results showed that the fragment was 62%-74% similarity to the cDNA sequences of pear other S-RNase alleles. A open reading frame comprised by 678bp encoding 226 amino acid was also found. Compared to DNA sequence of S42-RNase, the S42-RNase DNA sequence was spliced by two exons, one is 246bp, and the other 435bp,the deleted intron was 335bp and was stared with GT and ended with AG as usual.更多还原