【作者】 杨迎霞； 张颖； 郏艳红； 聂莉莉； 张越； 陈受宜； 王景安； 刘仲齐；

【Author】 YANG Ying-xia1) ZHANG Ying2) WANG Jing-an7) (Tianjin Normal University Tianjin 300384 ) JIA Yan-Hong3) NIE Li-Li4) ZHANG YUE5) LIU Zhong-qi8) (Tianjin Research Center of Agricultural Bio-technology Tianjin 300192) CHEN Shou-yi6)(Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101)

【机构】 天津师范大学； 天津市农业生物技术研究中心； 中国科学院遗传与发育生物学研究所；

【摘要】 以受体杨树107号(Populus×euramericana’Neva’)和转NTHK1基因杨树18-1的1年生枝条为材料,采用Hoagland水培的方法,对107和18-1植株生长量以及根和叶中Na+和K+的积累动态进行了研究,并对它们在盐碱地上的表现进行了调查。结果表明,18-1在轻度盐碱地上的生长速度显著高于107。在含0.6%NaCl的Hoagland溶液中,18-1的生长量显著大于107,9d后18-1叶片中Na+的浓度显著高于107。在叶片枯萎程度相同的情况下,18-1叶片中Na+含量是107的1.6倍左右。在4M NaCl溶液中,18-1和107表皮细胞的死亡率分别为42%和97%。盐胁迫条件下18-1叶片中NTHK1的转录水平显著高于107。说明NTHK1基因显著提高了杨树的耐盐潜力。更多还原

【Abstract】 Plant growth amount, change of Na+ and K+ concentrations in leaves and roots were investigated in 1-year-old cuttings from receptor poplar 107(Populus×euramericana ‘Neva’)and transgenic poplar 18-1 when they were cultured in Hoagland solution with or without NaCl. The growth speed of branches from 18-1 and 107 in saline soil was also tested. It was found that growth speed of 18-1 was much higher than that of 107 in light saline soil. The growth amount of 18-1 in Hoagland solution with 0.6% NaCl was also markedly greater than that of 107. After 9 day culture Na+ concentration in 18-1 leaves was greatly higher than that in 107. The accumulation amount of Na+ in 18-1 leaves was about 1.6 times higher than that in 107 when their leaves displayed same necrosis degree under NaCl stress.The death ratio of epidermal cells from 18-1 and 107 was about 42% and 97%, respectively. Relative transcript amount of NTHK1 in 18-1 leaves was much higher than that in 107 leaves when their plants were stressed with NaCl. These results indicated that introduction of NTHK1 remarkably improved the salt tolerance of poplar.更多还原