【作者】 李勇； 范立侨； 王子政； 赵群； 王冬； 刘羽； 李兆星；

【Author】 LI Yong;FAN Li-qiao;WANG Zi-zheng;ZHAO Qun;WANG Dong;LIU Yu;LI Zhao-xing;Department of General Surgery,the Fourth Affiliated Hospital,Hebei Medical University;

【机构】 河北医科大学第四医院外三科；

【摘要】 目的:观察胸导管淋巴引流与丹参多酚酸在重症急性胰腺炎并肝损伤的作用,为指导临床治疗提供实验依据。方法:大鼠随机分为假手术组(SHAM组)、颈部胸导管引流组(J组)、SAP(SAP组)、SAP+颈部胸导管引流组(Y组)、SAP+丹参多酚酸注射组(D组)、SAP+丹参多酚酸注射组+颈部胸导管引流组(YD组),每个模型组按时间点2小时、6小时、12小时,分为3个亚组(8只/组)。采用经胰胆管逆行注射5%牛磺胆酸钠法制备大鼠急性胰腺炎模型,检测大鼠血清中肝功能指标(ALT、AST)、淀粉酶(AMY),血浆及淋巴液内毒素含量,血清及肝脏过氧化氢酶及谷胱甘肽过氧化物酶活性及胰腺和肝脏的病理变化。结果:与S A P组比较,D组、Y组及YD组大鼠血清AMY、ALT、AST含量明显下降(P<0.05);血浆内毒素含量明显下降(P<0.05);血清及肝脏过氧化氢酶及谷胱甘肽过氧化物酶活性显著升高(P<0.05):显著改善胰腺和肝脏的病理损伤。结论:胸导管引流及静脉注射丹参多酚酸可减轻重症急性胰腺炎合并的肝脏损伤。更多还原

【Abstract】 Objective:To observe the thoracic duct lymph drainage and salvianolate in severe acute pancreatitis and liver injury,and provide experimental evidence for clinical treatment.The rats were randomly divided into sham group(SHAM group),cervical thoracic duct drainage group(J group),SAP(SAP group),SAP + cervical thoracic duct drainage group(Y group),SAP +salvianolate injection group(group D),SAP + salvianolate injection group + cervical thoracic duct drainage group(YD group),each model group by time point 2 hours,6 hours,12 hours,divided into three sub-groups(8 / group).Retrograde cholangiopancreatography is used by injection of 5%sodium taurocholate to make acute pancreatitis rats model,the content of hepatic function index(ALT,AST),the content of amylase(AMY) in serum,the content of endotoxin in plasma and lymph,the activity of catalase and glutathione peroxidase in serum and liver homogenate are examined,and the pathological changes in liver and pancreas were observed.Results:Compared with SAP group,the content of ALT,AST in serum,the content of endotoxin in plasma and lymph of Y group,D group and YD group significantly decreased(P <0.05),the activity of catalase and glutathione peroxidase in serum and liver of Y group,D group and YD group homogenate was significantly higher(P <0.05);the pathological lesions of the pancreas and liver were significantly improved.Conclusion:thoracic duct drainage and intravenous salvianolate relieve severe acute pancreatitis associated liver injury.更多还原