【作者】 吕晓楠； 王鹏； 白茹； 丛郢颖； 任晓峰； 陈春英；

【Author】 Xiaonan Lv;Peng Wang;Ru Bai;Yingying Cong;Xiaofeng Ren;Chunying Chen;National Center for Nanoscience and Technology of China;Department of Preventive Veterinary Medicine,College of Veterinary Medicine,Northeast Agricultural University;

【机构】 国家纳米科学中心； 东北农业大学；

【摘要】 猪传染性胃肠炎病毒(TGEV)是冠状病毒的一种,能导致仔猪严重腹泻,给养猪业造成重大的经济损失。虽然疫苗接种已经广泛用于预防猪传染性胃肠炎病毒的感染,但是免疫效果并不理想,这种疾病的传播依然存在[1]。因此开发能够预防和治疗猪传染性胃肠炎的药物迫在眉睫。近年来,银纳米材料由于具有优良的抗微生物特性,已逐渐成为人们关注的热点[2]。本研究以猪传染性胃肠炎作为冠状病毒模型,首次在体外评价了4种不同的银纳米材料(Ag NPs、Ag NWs 60、Ag NWs 400和silver colloids)对猪传染性胃肠炎病毒感染的宿主细胞及介导的细胞凋亡的抑制效果。通过MMP、p38 MAPK信号通路激活、Bcl-2家族蛋白的表达、半胱氨酸蛋白酶前体的降解等方面进一步探讨可能的分子机制。本研究为开发新型抗猪传染性胃肠炎病毒药物提供了参考。更多还原

【Abstract】 Coronaviruses belongs to the family Coronaviridae, which primarily cause infection of the upper respiratory and gastrointestinal tract of hosts. Silver nanomaterials(Ag NMs) have attracted great interests in recent years due to their excellent anti-microorganism properties. In this study, we used TGEV as a model of CoVs and comparatively evaluated the inhibitory effect of four different Ag NMs(Ag NPs, silver nanowires 60 nm, silver nanowires 400 nm and silver colloids)on TGEV-mediated cell infection and apoptosis. The underlying molecular mechanism was further explored. Mitochondrial membrane potential(MMP), p38 MAPK signal pathway activation, the expression of Bcl-2 family proteins and the cleavage of the precursor caspase-3 were measured. Our data indicate that Ag NMs are effective in prevention of TGEV-mediated cell infection as a virucidal agent or as an inhibitor of viral entry and the present findings may provide new insights into antiviral therapy of coronaviruses.更多还原