【作者】 胡燚； 张川； 贾儒； 邹彬； 徐娴；

【Author】 Yi Hu;Chuan Zhang;Ru Jia;Bin Zou;Xian Xu;College of Life Science and Pharmacy,Nanjing University of Technology;

【机构】 南京工业大学生物与制药工程学院；

【摘要】 通过引入功能化离子液体对介孔材料载体进行表面修饰,发展了一种脂肪酶固定化新方法,酶固定化效率、活性、热稳定性、PH耐受性、操作稳定性等都得到了显著提高;通过CD、FT-IR、TEM、TG、TPD、BET、SAXRD、NMR等方法对修饰前后载体和酶蛋白空间结构变化进行了解析,探索了酶学性质改善的分子机制。通过理性设计功能性离子液体的分子结构,对酶分子序列中的特定氨基酸残基进行定向共价修饰,可以同时显著提升PPL、CAL-B、CRL等多种脂肪酶的活性、热稳定性、选择性、有机溶剂耐受性等催化性能;并通过UV、FS、CD、MALDI-TOF-MS等手段进行结构表征,结合分子模拟手段对修饰机理进行了合理解析,发展了一种功能性离子液体化学修饰脂肪酶分子改造的新方法。更多还原

【Abstract】 A series of functional ionic liquids were incorporated into the surface of mesoporous materials. This research work exploited a novel and efficient method to modify the surface of mesoporous materials for lipases immobilization. The modified enzymes showed significant improvements in enzyme immobilization efficiency, activity, thermal stability, pH tolerance, and operating stability. Immobilized enzymes were characterized by CD, FT-IR, TEM, TG, TPD, BET, SAXRD, NMR, etc. to analyze the conformational changes of carriers and proteins to evaluate the mechanism of enhanced catalytic properties. Various functional ionic liquids were rationally designed and chemically modified with specific amino acid residues of lipases. Simultaneously significant enhancement of activity, thermal stability, selectivity, and organic solvent tolerance were achieved for some lipases such as PPL, CALB, CRL, etc. Reasonable analysis to the modification mechanism were obtained from UV、FS、CD、MALDI-TOF-MS as well as molecular simulation. We aim to develop a novel, efficient and practical method to enhance the catalytic performance of lipases.更多还原