【作者】 葛晓晓； 刘碧荣； 张爱东； 杜丹；

【Author】 Xiaoxiao Ge;Birong Liu;Aidong Zhang;Dan Du;Department of Chemistry, Centra China Normal University;

【机构】 华中师范大学化学学院；

【摘要】 p53蛋白是机体细胞内一种重要的抗癌蛋白,在细胞增殖,DNA修复等过程中有着极为重要的作用。当受到外界环境刺激时,该蛋白的一些氨基酸位点会发生异常的磷酸化,从而引起细胞癌变。[1]因此对磷化蛋白p53快速、灵敏、有效的检测对癌症早期诊断及治疗有着重要的意义。免疫传感器灵敏度高、选择性好、线性范围宽,是一种高效、快速的定量检测手段。[2]本文以p53蛋白丝氨酸15号位点的磷酸化蛋白(p53S15)做为待测物,Fe3O4作为纳米载体,Ferritin作为信号标记物,构建了一个新颖的免疫传感器,利用磁纳米颗粒和Ferritin的类酶性质,催化H2O2和TMB显色,放大了检测信号,从而实现对Phospho-p53S15高灵敏的定量检测。更多还原

【Abstract】 As a very important anti-cancer protein, p53 plays a key role in cell proliferation and DNA repair progress. When exposure to external environment stress, p53 can lose its normal, which may result in the cancer. Thus, it is critical to early clinical diagnosis and treatment of cancer. Due to the high sensitivity and selectivity, the immunosensor can be used as an efficient method for detection of some biomarkers. In this paper, we develop a novel immunosensor based on HRP-like property of Fe3O4 and Ferritin for p53S15 assay, in which the Fe3O4 and Ferritin can simultaneously catalyzed H2O2 and TMB to produce color change.更多还原