【作者】 张柏林； 唐纪琳； 侯慧； 白效静；

【Author】 Zhang Bailin;Tang Jilin;Hou Hui;Bai Xiaojing;State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry,Chinese Academy of Sciences;University of Chinese Academy of Sciences;

【机构】 中国科学院长春应用化学研究所； 中国科学院大学；

【摘要】 作为一种响应速度快、灵敏度高且无需标记的新型传感器,近年来,微悬臂传感器已发展为一种非常有前景的生物传感技术1,2。微悬臂传感器有两种工作模式:静态模式和动态模式。在静态模式下,待测物与固定在悬臂一侧表面的识别分子相互作用,使悬臂上下两个表面出现应力差从而产生偏转。悬臂偏转的大小和待测物的浓度在一定范围内相关。我们将适配子(或单链DNA)作为特异性的识别分子通过Au-S键修饰在悬臂金表面,通过测定悬臂的偏转,分别实现了土霉素、卡那霉素和单链结合蛋白的无标记检测。另外,利用多肽修饰的微悬臂传感器,我们发展了一种可逆性的万古霉素检测方法。我们的工作展示了微悬臂传感器在生物分子识别和检测方面的巨大应用潜力。更多还原

【Abstract】 As a rapid, highly sensitive and label-free detection approach, cantilever sensors have become an emerging and promising technology for biosensing applications 1, 2. They can be operated in static mode or dynamic mode. In static mode, binding of analytes and recognition molecules immobilized on one surface of the cantilever causes a change in cantilever surface stress and deflects the cantilever. The deflection is influenced by the concentration of the analytes over a certain range. We immobilized DNA aptamers or single-stranded DNA(ssDNA) on the gold surface of the cantilevers. Relying on resolving cantilever deflections, we developed label-free detection methods for oxytetracycline(OTC), kanamycin and single-stranded DNA-binding protein(SSBP), respectively. Furthermore, utilizing the peptide-modified cantilever array sensor, we developed a reversible detection method for vancomycin. Our results show the great potential of the cantilever sensor system as a powerful tool in recognition and detection of biomolecules..更多还原