【作者】 张璐； 张翼； 孙佳姝； 蒋兴宇；

【Author】 Lu Zhang;Yi Zhang;Jiashu Sun;Xingyu Jiang;Beijing Engineering Research Center for BioNanotechnology & Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology;Department of Biomedical Engineering, College of Engineering, Peking University;

【机构】 国家纳米科学中心； 北京大学；

【摘要】 本文建立了基于毛细管的核酸等温扩增反应(cLAMP)方法,实现了直观、稳定、多重和即时的核酸分析。该方法集成了玻璃毛细管或塑料毛细管,可引入样品和反应试剂液滴,并利用水滴来隔离不同样品和防止污染,通过使用微型加热器提供热量完成反应,并利用手持式手电筒进行荧光信号的可视读出。该系统可实现多个血浆样品中人类免疫缺陷病毒(HIV)的两个靶标RNA的同时检测,对质粒样品的检测灵敏度可达两个拷贝。该核酸检测方法完全独立于外部电源和设备,在资源贫乏地区的即时检测方面具有广阔的应用前景。更多还原

【Abstract】 This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification(cLAMP) for assaying nucleic acids. This assay integrates capillaries(glass or plastic) to introduce and house sample/reagents, segmentsof water droplets to prevent contamination, pocket warmers to provide heat, and a handheld flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus(HIV) from multiple plasma samples, and achieves a high sensitivity of 2 copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings.更多还原