【作者】 韩疏影； 梁超； 乔俊琴； 练鸿振； 葛欣；

【Author】 S.Y.Han;C.Liang;J.Q.Qiao;H.Z.Lian;X.Ge;College of Pharmacy, Nanjing University of Chinese Medicine;State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University;

【机构】 南京中医药大学药学院； 生命分析化学国家重点实验室,南京大学化学化工学院和现代分析中心；

【摘要】 本文讨论了流动相中的正辛醇对物质的表观正辛醇-水分配系数(logKow′′)与外推至纯水的反相液相色谱(RPLC)保留值(logkw(o))之间QSRRs关系的影响。选取11种苯甲酸类和15种苯胺类+吡啶类化合物为研究对象,建立了不同离子抑制剂下其相应的QSRRs关系,还讨论了正辛醇对物质的保留行为(k)与有机调节剂比例(φ)之间的关系,以及对RPLC保留机理的影响。结果表明,在酸性化合物体系中,若以高氯酸(强酸)作为离子抑制剂,溶质与固定相残留硅羟基之间的作用可忽略不计,此时正辛醇/水分配过程与色谱过程是均能的,正辛醇只起有机调节剂的作用;若以乙酸(弱酸)或磷酸/磷酸二氢钾(缓冲盐)作为离子抑制剂,正辛醇不仅是有机调节剂,还是残存硅羟基的掩蔽剂。然而,在碱性化合物体系中,以氯化铵-氨水为离子抑制剂时,正辛醇的加入则会使QSRRs相关性显著变差。因此,在研究化合物的保留行为及相应的QSRRs关系时,正辛醇只适合用于酸性体系,且推荐以强酸,如高氯酸作为离子抑制剂。更多还原

【Abstract】 The influence on the quantitative structure-retention relationships(QSRRs) of lipophilicity and retention behavior of weak acids and bases in reversed-phase liquid chromatography(RPLC) by adding n-octanol into mobile phase was discussed in this paper, especially for the QSRRs of logarithm of apparent n-octanol/water partition coefficient(logKow′′) and logarithm of retention factor extrapolated to neat aqueous mobile phase(logkw(o)). The results indicated that the roles of n-octanol were different when various ion-suppressors were used in acidic or basic systems. For studying on QSRRs of lipophilicity and retention behavior of acidic compounds, strong monoprotic acids, e.g., perchloric acid, are recommended as the ion-suppressors, while it seems that addition of n-octanol is not suitable in basic systems.更多还原