【作者】 徐向东； 李丽萍； 刘慧敏； 石红梅； 康维钧；

【Author】 Xiangdong Xu;Liping Li;Huimin Liu;Hongmei Shi;Weijun Kang;School of Public Health, Hebei Medical University;

【机构】 河北医科大学；

【摘要】 绿原酸、阿魏酸、香草酸和咖啡酸这几种酚酸类化合物是存在于天然药物中的有效成分,具有清除自由基、抑制血栓、抗菌消炎、抑制低密度脂蛋白的氧化等生物活性。目前,其检测方法主要包括液相色谱法、液相色谱-质谱联用等,但上述方法普遍分析成本较高,有些还需复杂的样品前处理或衍生化。实验发现绿原酸、阿魏酸、香草酸和咖啡酸加入Ag(Ⅲ)配合物-鲁米诺化学发光体系时,可使化学发光信号强烈降低,且降低的程度与其浓度呈比例关系。利用自制的毛细管电泳-化学发光检测仪,在优化的实验条件下,四种酚酸的检出限可低至0.05~0.1mg/L。结合固相萃取技术,可实现枸杞、双黄连口服液、蒲公英颗粒等中药材和中成药中绿原酸、阿魏酸、香草酸和咖啡酸的检测。更多还原

【Abstract】 Chlorogenic acid, ferulic acid, vanillic acid and coffee acid are effective components in natural medicines. A novel method for the analysis of the four phenolic acids was developed by capillary zone electrophoresischemiluminescence method based on the fact that the four they could quench the chemiluminescence reaction of luminol-Ag(Ⅲ) system, and the extent of reduction was proportional to their concentration. Under the optimum conditions, the detection limits of the four phenolic acids can be as low as 0.05 ~ 0.1mg/L. Combined with solid phase extraction, the presented method was successfully applied to the determination of chlorogenic acid, ferulic acid, vanillic acid and coffee acid in some Chinese herbal medicines and proprietary Chinese medicines.更多还原