【作者】 史聪颖； 梁家杰； 周克楠； 付强强； 向军俭； 唐勇；

【Author】 CongYing Shi 1,JiaJie Liang 1,KeNan Zhou 1,QiangQiang Fu 1,Junjiang Xiang 1,2,Yong Tang 1,2 * 1.Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering,Jinan University,Guangzhou 510632,PR China 2.Institute of translational medicine,Jinan University,Guangzhou 510632,PR China

【机构】 暨南大学广东省分子免疫学与抗体工程重点实验室； 暨南大学转化医学研究院；

【摘要】 用于小分子检测的免疫竞争胶体金试纸条使用时,高浓度的阳性样品使检测线信号完全消失,且所得检测信号与样品浓度呈负相关,易引起误判。为了克服这个问题,我们在胶体金试纸条的平台上,根据荧光共振能量转移(FRET)的原理,建立了一种新型的荧光免标记猝灭试纸条。该方法制备更简便,更易于引起荧光共振能量转移,产生荧光猝灭,可转换检测信号和提高灵敏度。在本研究中,我们以莱克多巴胺为检测目标建立了荧光免标记猝灭试纸条,该试纸条对猪尿中的莱克多巴胺的最低检测限可达到0.31ng/mL,且荧光信号与样品浓度正相关。该方法对比胶体金试纸条有较多的优势,如更灵敏,得到的结果数值与样品浓度呈正相关,可定量等,其设计思路可适用于试纸条平台上的各种小分子检测研究。更多还原

【Abstract】 When traditional immunochromatographic lateral flow assay(LFA) strips were used to detect small molecule analyte,only the concentration of small molecules in samples reaches or exceeds a certain concentration exactly,the visualized signal will disappear completely.The test signal is negative related to analyte concentration which may easily mislead users.In order to overcome this disadvantage,a novel fluorescence quenching lateral flow sensor was developed based on the principle of FRET and competitive immunoassays.The fluorescence quenching LFA(FQLFA) strips are positive readout and more sensitive.The FQLFA strips can quantitatively analyzed the Rac in phosphate buffer(PB) and swine urine according to this research.The limit detection of the developed strips for Rac in swine urine is 0.31 ng/mL by using fluorescence LFA strip reader.The sensitivity of this LFA is more sensitive than the latest reported gold base LFA strips of Rac.This developed novel scheme is much suitable for small molecules rapid detection.更多还原