【作者】 朱晓娜； 曹伟伟； 朱金花； 李明静；

【Author】 Xiaona ZHU 1,weiwei CAO 1,jinhua ZHU 1,Mingjing LING 1,2 1 College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004;2 Key Lab of Natural Drug and Lmmune Engineering,Henan Province,Kaifeng 475004

【机构】 河南大学化学化工学院； 河南大学河南省天然产物与免疫工程重点实验室；

【摘要】 <正>应用高速逆流色谱法,分离制备槐花中的黄酮类化合物。以正己烷-乙酸乙酯-乙醇-水-冰醋酸为两相溶剂体系,应用高速逆流色谱法进行分离,并考察了在上述两相溶剂体系中加入一定量的离子液体来优化分更多还原

【Abstract】 Two flavones were prepared,isolated and purified from Sophora japonica by high-speed countercurrent chromatography(HSCCC) for the first time using ionic liquids to improve the separation effect.A two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water-acetic acid and ionic liquid was used.The obtained fractions were analyzed by HPLC to test its purity and to ensure the compound.The results indicated that 18.2 mg of rutin and 9.6 mg of quercetin with their purities over 97 % were obtained from 50 mg crude extract of Sophora japonica.By adding ionic liquid,the retention time of the compound decreased from 80 min to 50 min,and the resolution increased from 0.9 to 1.8.The separation effect was significantly improved and complete separation was achieved.The established method can be used for the separation and purification of flavones from the Sophora japonica.更多还原