【作者】 余欢； 李继山； 杨盛； 杨荣华；

【Author】 Huan Yu,Jishan Li,Sheng Yang,Ronghua Yang State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha,410082.

【机构】 湖南大学化学化工学院化学生物传感与计量学国家重点实验室；

【摘要】 <正>通过加入分析物来催化有机反应得到荧光产物是很好的检测生物分子的方法。Staudinger反应是发生在膦化合物和叠氮化合物之间的还原反应,得到产物胺和膦的氧化物1。这个反应有很好的生物相容性且易于更多还原

【Abstract】 Analyte addition and catalyze the organic reaction which leading to a fluorescence product represent an attractive strategy for the detection of biomolecules.The Staudinger reaction is between a phosphine and an azide and give a primary amine and the corresponding phosphine as product.The reaction is biocompatible and orthogonal to most biological functionalities.We synthesize a 7-azidocoumarin whose fluorescence is quenched by introduce an azide group.Upon addition of triphenylphosphine(TPP),the Staudinger reaction take place and fluorescence recover on mM concentration.We report the application based on an ATP assisted sandwich panel structure which draw the distance between two divided ATP aptamer coujugated with 7-azidocoumarin and TPP,then catalyze the Staudinger reaction under lower concentration and get fluorescent 7-aminocoumarin as signal product for the detection of ATP.更多还原