【作者】 陶佳； 李银辉； 段玉； 李继山； 杨荣华；

【Author】 Jia Tao,Yinhui Li,Yu Duan,Jishan Li,Ronghua Yang State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha,410082

【机构】 湖南大学化学化工学院化学生物传感与计量学国家重点实验室；

【摘要】 <正>本文设计合成了一种基于光诱导信号变化的螺吡喃探针用于水解酶活性检测。该探针由两部分组成,螺吡喃和β-半乳糖,经过光照开环的探针在水解酶的作用下,开环体部花菁可形成对苯二酚的结构,可以更多还原

【Abstract】 The paper has designed new spiropyran-based electrochemical probe,SP-β-D-glucose,to identify the glycosidases based on photo induced comformational change.The probe was fixed on the electrode on the basis of the affinity between the SWNT and the probe resulted from the electrostatic and conjugate interactions.This strategy offers a sensitive,specific,and efficient platform for quantitatively screening the glycosidases digestions.With the electrode as the assay platform,the technique also creates a new dimension for analysis of glycosidase.更多还原