【作者】 彭娟； 姜立萍； 朱俊杰；

【Author】 Juan Peng,Li-Ping Jiang,and Jun-Jie Zhu School of Chemistry and Chemical Engineering,Nanjing University,Nanjing,210093

【机构】 南京大学化学化工学院；

【摘要】 <正>本文围绕球形纳米复合材料的构建及其在免疫传感中的应用开展研究。通过模板法制备了一种新颖的银纳米粒子-磷酸钛空心球(AgNPs-TiP)复合材料,该复合材料具有良好的生物相容性、分散性及高的银更多还原

【Abstract】 In this paper,we studied the synthesis of spherical nanocomposites and their applications for the fabrication of electrochemical immunosensors.A novel nanocomposite silver nanoparticle-titanium phosphate hollow sphere(AgNPs-TiP) was synthesized by a template approach and used for assembly of antibodies as a biolabel for high sensitive electrochemical immunoassay of human IL-6.A multifunctional manganese carbonate sphere(MnCO 3) with superparamagnetic and fluorescent properties was prepared through co-precipitation and layer by layer methods.Based on the multifunctional MnCO 3 labelled with antibodies as probe,a versatile immunosensor for fluorescent imaging and electrochemical detection of AFP was developed.Fluorescent gold nanoclusters were incorporated into porous calcium carbonate sphere to fabricate CaCO 3 /AuNCs nanocomposite.The CaCO 3 /AuNCs showed porous structure,good biocompatibility,excellent dispersity and degradability,which can be used to assembly of HRP-Ab 2 for fabrication a biolabel.Based on this biolabel,a multifunctional immunosensor was fabricated for fluorescent and electrochemical detection of cancer biomarker with high sensitivity and selectivity.更多还原